Protein Info for GFF5899 in Variovorax sp. SCN45

Annotation: Phosphoglycolate phosphatase (EC 3.1.3.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 233 PF00702: Hydrolase" amino acids 12 to 199 (188 residues), 106.4 bits, see alignment E=5.1e-34 PF12710: HAD" amino acids 14 to 196 (183 residues), 33 bits, see alignment E=1.7e-11 PF13419: HAD_2" amino acids 15 to 205 (191 residues), 116 bits, see alignment E=4.5e-37 TIGR01449: phosphoglycolate phosphatase, bacterial" amino acids 15 to 232 (218 residues), 201.6 bits, see alignment E=1.7e-63 TIGR01549: HAD hydrolase, family IA, variant 1" amino acids 79 to 199 (121 residues), 43.3 bits, see alignment E=7.7e-15 TIGR01509: HAD hydrolase, family IA, variant 3" amino acids 95 to 205 (111 residues), 35.2 bits, see alignment E=1.9e-12 PF13242: Hydrolase_like" amino acids 161 to 230 (70 residues), 47.5 bits, see alignment E=2.8e-16

Best Hits

KEGG orthology group: K01091, phosphoglycolate phosphatase [EC: 3.1.3.18] (inferred from 86% identity to vpe:Varpa_0626)

Predicted SEED Role

"Phosphoglycolate phosphatase (EC 3.1.3.18)" in subsystem 2-phosphoglycolate salvage or Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 3.1.3.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.18

Use Curated BLAST to search for 3.1.3.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (233 amino acids)

>GFF5899 Phosphoglycolate phosphatase (EC 3.1.3.18) (Variovorax sp. SCN45)
LNSISLHPHRFDAAIVDLDGTMVDTLGDFAASLNRMLADLSLPAVEPAAIERMVGKGSEH
LIHSALVHVMAPTEGNAADARARALFDRAWERYQHHYLAVNGQHSAVYAGVIDGLKALRA
QGLRLACLTNKPTSFAKPLLAEKGLDGFFELVFGGDAFERKKPDPLPLLKTCEALGTTPA
RTLMVGDSSNDARAARAAGCPVVLVTYGYNHGEPVRAVDADGFVDSLAELAAA