Protein Info for GFF5822 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Nitrate/nitrite sensor protein (EC 2.7.3.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 638 transmembrane" amino acids 13 to 36 (24 residues), see Phobius details amino acids 160 to 186 (27 residues), see Phobius details PF13675: PilJ" amino acids 33 to 131 (99 residues), 83.9 bits, see alignment E=2.2e-27 PF00672: HAMP" amino acids 185 to 232 (48 residues), 45.6 bits, see alignment 1.8e-15 PF01590: GAF" amino acids 272 to 407 (136 residues), 37 bits, see alignment E=1.2e-12 PF07730: HisKA_3" amino acids 424 to 490 (67 residues), 59.7 bits, see alignment E=8.6e-20 PF02518: HATPase_c" amino acids 531 to 620 (90 residues), 49.3 bits, see alignment E=1.6e-16

Best Hits

KEGG orthology group: K07673, two-component system, NarL family, nitrate/nitrite sensor histidine kinase NarX [EC: 2.7.13.3] (inferred from 74% identity to pol:Bpro_4599)

Predicted SEED Role

"Nitrate/nitrite sensor protein (EC 2.7.3.-)" in subsystem Nitrate and nitrite ammonification (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (638 amino acids)

>GFF5822 Nitrate/nitrite sensor protein (EC 2.7.3.-) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VAMVSTASLSTKLVRIGAGLLLVALASIGLTLWVTWQLEGGAAAVNEAGRMRMQTWRLTS
VAQAQRAPAEVAALVQRFDQSLALLRRGDASRPLFVPRDEAVAREFATVEALWQKQRPLW
LQDTPPEPAQVLAAADTFVEAIDALVLAIEHQLSAFTAILNLFQFLMMALAIVGAVVMLY
TGYMYVINPLAHLRQGLRRLESGDFKARVEVDTLDEFGQVAGGFNRMASTLQSMYAGLES
QVETKTRHIEAQRARLATLYEVSAFMAQAGSIEELSRGFAQRVRAVVKADAVAVRWSDEA
SQRYLMLASDCFPQELVEEERSLLAGACACGSLQPDARTRVIPIHSHDEASVRHCARAGF
ETLVSVPVRLQNRLLGEIDLFYRSQASLSTGETELLDALASHLANALESLRASALEREAA
VGEERALIARELHDSIAQSLAFLKIQVQLLRTAAGKGQDARVMSTLDELDEGLRESINDV
RELLVHFRTRTNTDDIERALQETLQKFQHQTGLPAKLHVEGHGLPLPADVQVQVLHVLQE
SLSNVRKHAGAGHVQLDVFKGAHWRFRVCDDGAGFDALDASDDSHVGLKIMRERAALIGA
RVDVSSDPGQGTAVTLTLPPHPVTARPSGAPVTEHRLS