Protein Info for GFF582 in Variovorax sp. SCN45

Annotation: Two-component system sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 515 transmembrane" amino acids 23 to 42 (20 residues), see Phobius details amino acids 202 to 222 (21 residues), see Phobius details PF08521: 2CSK_N" amino acids 34 to 189 (156 residues), 60.4 bits, see alignment E=3e-20 PF00512: HisKA" amino acids 270 to 332 (63 residues), 37.5 bits, see alignment E=2.9e-13 PF02518: HATPase_c" amino acids 398 to 513 (116 residues), 78.4 bits, see alignment E=8.8e-26

Best Hits

KEGG orthology group: K07649, two-component system, OmpR family, sensor histidine kinase TctE [EC: 2.7.13.3] (inferred from 84% identity to vpe:Varpa_3539)

Predicted SEED Role

"Tricarboxylate transport sensor protein TctE"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (515 amino acids)

>GFF582 Two-component system sensor histidine kinase (Variovorax sp. SCN45)
MDPQPQPPTPSPATEPQRFGIRTRLLALLLPGMVTLLALDSWNDYHALTDSLVAAYDQSL
LEPVQALANGIVVASDGSVRVQEPFSIQAMFESTHLRYKYLHVGVQRIPKGETLDVNSPE
NTLMGVPGLPRPATRPADGVPVFYDAVHEQHRVRVAALRQTVYDHVHPGVAYSVLIQAAE
GIGPRTEAQSESLRQELLRDTRMVLVMALLVWLGVAWTLRPLERLRRSLRERSRDDLKPL
DANGVPKEVVPLVDAVNHHIASHRRMVEEQSQFLADASHQLRTPLSILSIQAGYAVRETD
PARMRESLHAIVAQLARTRRLSEQLLALAHATTEGEAARAEPAVVDLNAIARGVVLEYLP
LARENDQDLGWVDARSDTAAGDDAEDDDTPVLPVLANAAELHEVLANLVHNALKYTPRGG
NITVTVRREASSALAEVCDNGPGVAPERRESVFERFHRDPAVDAGAAHGAGLGLTIARGY
ARRNGGEIELDSAGMPESNTGGGLRATLRLPLHLG