Protein Info for GFF5818 in Variovorax sp. SCN45

Annotation: Two-component transcriptional response regulator, NarL/FixJ family

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 211 PF00072: Response_reg" amino acids 9 to 120 (112 residues), 92.1 bits, see alignment E=2.6e-30 PF00196: GerE" amino acids 147 to 201 (55 residues), 58.8 bits, see alignment E=3.4e-20

Best Hits

Swiss-Prot: 38% identical to UHPA_ECOL6: Transcriptional regulatory protein UhpA (uhpA) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: None (inferred from 91% identity to vap:Vapar_3992)

MetaCyc: 32% identical to DNA-binding transcriptional dual regulator NarL (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"DNA-binding response regulator, LuxR family"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (211 amino acids)

>GFF5818 Two-component transcriptional response regulator, NarL/FixJ family (Variovorax sp. SCN45)
MSTQAIRLFLVDDHPLVRDGLRARLGSMPNLEIVGEAGSAAEALAQIDSVRPDLMLMDVG
MKDMNGIELAALLLQRQGAPHIVMLSMYDNPEYVQKALQAGARGYVLKDAPAAEIVAAIE
AVSAGGTFLSPAVSKKLFRTQEPRPLLTPRESEILSALGRGESSKQIARDLGLSVRTVEA
HRQSIKRRLGIEGQAELIKYAVEHARKFGQS