Protein Info for GFF5817 in Variovorax sp. SCN45

Annotation: Two-component system sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details transmembrane" amino acids 203 to 226 (24 residues), see Phobius details PF17200: sCache_2" amino acids 47 to 190 (144 residues), 133.3 bits, see alignment E=1.9e-42 PF17201: Cache_3-Cache_2" amino acids 74 to 192 (119 residues), 49.2 bits, see alignment E=1.2e-16 PF08269: dCache_2" amino acids 74 to 184 (111 residues), 115.2 bits, see alignment E=8.4e-37 PF07730: HisKA_3" amino acids 250 to 316 (67 residues), 45.9 bits, see alignment E=1.7e-15 PF02518: HATPase_c" amino acids 357 to 449 (93 residues), 44.7 bits, see alignment E=4.1e-15

Best Hits

KEGG orthology group: K02480, two-component system, NarL family, sensor kinase [EC: 2.7.13.3] (inferred from 87% identity to vpe:Varpa_4636)

Predicted SEED Role

"Sensor histidine kinase"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (457 amino acids)

>GFF5817 Two-component system sensor histidine kinase (Variovorax sp. SCN45)
MNLRTKIVALAVAPLLIALVLVALAVRHQEHDLAQRERALIERSYMAQRRGELRSYVDLA
VSNLLPLYESGKDDEATRNEALRRLAALNYGDDGYFFVYDLEGNSLMHSRQPELVGQNLW
GMRDSHGRYTIQELIKGAKEKGGAYVEYEWRKPSSAQLAPKLGYVTAMPRWNWMVGTGLY
LDDIQSTMDTLDRQMNANVTATLLWIAGIAALCLGVVSAAGLLLNLSEHRTAEAKLRLLA
RRVVQSQEEERGHLARELHDGTSQTLVSAKLLIESAVESLESRHQPTPPALPKALQRLND
SLIEVRRISHRLRPALLDTLGLPAALERLVDEFREEGAVDASMMIEGEAFELQPEVKTAL
FRLTQEALTNVRKHANAQHVHVALAFDEKDGVRLEVSDDGTGFDIEAVQLDPRRGIGLRN
MRERMEAIGGQLIMCSNEGRTSIEAEVPARNARAASA