Protein Info for GFF5813 in Variovorax sp. SCN45

Annotation: Heavy metal sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 465 signal peptide" amino acids 1 to 34 (34 residues), see Phobius details transmembrane" amino acids 157 to 181 (25 residues), see Phobius details TIGR01386: heavy metal sensor kinase" amino acids 2 to 456 (455 residues), 411.9 bits, see alignment E=1.9e-127 PF00672: HAMP" amino acids 179 to 232 (54 residues), 34.2 bits, see alignment 5.4e-12 PF00512: HisKA" amino acids 237 to 302 (66 residues), 56.7 bits, see alignment E=4.2e-19 PF02518: HATPase_c" amino acids 351 to 454 (104 residues), 75.4 bits, see alignment E=9.5e-25 PF14501: HATPase_c_5" amino acids 354 to 445 (92 residues), 26.1 bits, see alignment E=1.4e-09

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 84% identity to vpe:Varpa_4640)

Predicted SEED Role

"Signal transduction histidine kinase"

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (465 amino acids)

>GFF5813 Heavy metal sensor histidine kinase (Variovorax sp. SCN45)
VKRSITVRLVIMFAAVALATFALAGVALHNVLARELERHQYEELDTTLKNLQFWIKAIGS
PERWSRVQARMDALTPADGSVRFWVLSDDPRFQYGKGLAEIDGLTREANGHSSIIELPGQ
ERPFRTLSAHIDPFEQRPAVRLIVGHNTEPYARTRQAFLTALIGLGLGAVLVVAVAGYWI
ARVGLRPLERLSREAQALRPKTLSHRLRSEPLPVELSALAEAFNGALSRLEEAYGQLEAF
NADVAHELRTPLANLIGSTQVALSRQRSAGEFQEVLQANLEDLERVRSIVNDMLFLARAD
RGEVATGLVLTPVAQEVRKTIEFFEFVLDETRAQVSIEGDVHTEARLESALFRRAMSNLL
QNAIEHSEPGAHIVVMLREEEAATWITVSNPGAPIDAQHMPHLFDRFYRADASRNDRGEH
HGHGLGLAIVKAVASMHGGQVSASRQGGLNTFGFSVARTPGNPAG