Protein Info for GFF58 in Variovorax sp. SCN45

Annotation: Tyrosine-protein kinase (EC 2.7.10.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 754 transmembrane" amino acids 24 to 43 (20 residues), see Phobius details amino acids 438 to 459 (22 residues), see Phobius details PF02706: Wzz" amino acids 13 to 101 (89 residues), 47.8 bits, see alignment E=3e-16 TIGR01005: exopolysaccharide transport protein family" amino acids 13 to 733 (721 residues), 425.6 bits, see alignment E=3.8e-131 PF13807: GNVR" amino acids 382 to 461 (80 residues), 90.6 bits, see alignment E=1e-29 TIGR01007: capsular exopolysaccharide family" amino acids 521 to 722 (202 residues), 135 bits, see alignment E=2.5e-43 PF01656: CbiA" amino acids 542 to 713 (172 residues), 33 bits, see alignment E=1.1e-11 PF13614: AAA_31" amino acids 545 to 693 (149 residues), 45.9 bits, see alignment E=1.3e-15

Best Hits

Swiss-Prot: 44% identical to EPSB_RALSO: Putative tyrosine-protein kinase EpsB (epsB) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: None (inferred from 71% identity to vpe:Varpa_2883)

Predicted SEED Role

"Tyrosine-protein kinase Wzc (EC 2.7.10.2)" in subsystem Colanic acid biosynthesis (EC 2.7.10.2)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.10.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (754 amino acids)

>GFF58 Tyrosine-protein kinase (EC 2.7.10.2) (Variovorax sp. SCN45)
MVEAAWVRESRFQEYLDILIDSRWLIAGLTALALVAGAGYALFGPRVYEANILIQVEDSD
RSGGNFPGDATGGAGISVKTPVAGETEILRSRMVLGQAMENTRLYISAKPRYIPVVGEFL
ARHSNRLSTPGIFGMGDYVSGTERIAVAQMDVPQPLEGKKFLLTAEADGNYTLTNSGLEA
PLQGKVGVPLDAAVPGGTLHLLVGSFEAEPGAGFELVRYSKQLTLLGLQKDLRVVEKGKQ
SGVMDVSLQAENRGQLADVLNEISRLYVRQNIDQKTVQAERALAFLGTELPKFKAQLEQS
EDSYNQYRNQNGTISLDDEARNALSQNVDLQAKLLDAKQKRLDLVGRFTAAHPAVQTLDS
QIASLKKELGAVDGRIRRMPMLQQNSLRMQRDIKVNTDLYATLLNSSLQMRLAKEGKVAN
VRVLDQALLPEKPVRPKALIVMALSLAGGLFLGGATALLRKTLKKSIGSPDEIEAVTGLN
VYSTIALSTQQRVLDRAIRGGKPGVKVLAALHVEDPALEGLRRLRTALKFVMLGAPNNRV
LISSATPGAGKTFVSSNFATLVASSGKRVLLIDADLRRGTLSEEFGLQRKDGLSDVITGG
VPLDRAIHYHVLPRLDVMTSGTLHPDPAGMMTSDVFAQTLATLSSRYDVVIVDAPPTLLA
SETAAMAPSMGTLLLVARADHSESGELLESVKRLGHVGAEFHGVVFNAVDTTQRHYRGYS
YGYYDAYRREPAGSLGVNDADTVMPKRLEPEHAA