Protein Info for GFF5763 in Variovorax sp. SCN45

Annotation: Signal transduction histidine kinase CheA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 698 PF01627: Hpt" amino acids 7 to 102 (96 residues), 65 bits, see alignment E=1.5e-21 PF09078: CheY-binding" amino acids 172 to 234 (63 residues), 82.9 bits, see alignment E=3.5e-27 PF02895: H-kinase_dim" amino acids 293 to 356 (64 residues), 75 bits, see alignment E=1.3e-24 PF02518: HATPase_c" amino acids 404 to 540 (137 residues), 63.8 bits, see alignment E=4.9e-21 PF01584: CheW" amino acids 546 to 672 (127 residues), 100 bits, see alignment E=2.2e-32

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 82% identity to vap:Vapar_4156)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (698 amino acids)

>GFF5763 Signal transduction histidine kinase CheA (Variovorax sp. SCN45)
MDLSQFTQAFFVEAIELLAEMEQLLLDLDVEAPDSEQLNAIFRAAHSIKGGAATFGFTAL
TNTTHVLETLLDRARHGQLSLNGRMIDAFLETKDALQEQLTAYQAGQEPNPDMVAHICGV
LQQLALESGDESLAAHAAATAAAAPVAAPAPAPVAAAAPAEAAVPAGDGVLRIRFARLSD
SECDLLAGELSNLGKVLSRTRSNDQLTVLLETTCDPDDIVAVCCFVIDESQIDITREAAA
AVVAPEPAAPAPAAEAPAKAPAVVAAAAPVAQAAPAAPAATAAPAAAAGTKESSSIRVDV
EKVDQLINLVGELVITQSMLTQAATMLDPVAYERFLSGLGHLERNARDLQESVMSIRMMP
MDYVFSRFPRVIRDVSAKLGKQVRLDTYGKETELDKGLIERIVDPLTHLVRNSLDHGIET
PDVRLAKGKDATGQLLLSAQHHGGNIVIEVSDDGAGLNRERILAKALQQGLPVSETMPDE
EVWQLIFAPGFSTAEQVTDISGRGVGMDVVKRNIQEMGGHVEIHSRGGQGTTTRIVLPLT
LAILNGMSVKVGDEAYILPLSYVIESLQPLPEHLHSITADGHVIRVRGEYLPLIELHRVF
DVAGAQTHPTQGILVIVQADDSRFALLVDELLGQHQVVVKNLETNYRKVPGISAATILGD
GSVAFIIDVGAMPRIQRAQAASAAALAGAARRTDAIAL