Protein Info for GFF5750 in Variovorax sp. SCN45

Annotation: Flagellar M-ring protein FliF

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 578 transmembrane" amino acids 30 to 49 (20 residues), see Phobius details amino acids 486 to 504 (19 residues), see Phobius details TIGR00206: flagellar M-ring protein FliF" amino acids 20 to 577 (558 residues), 414.5 bits, see alignment E=4.5e-128 PF01514: YscJ_FliF" amino acids 51 to 225 (175 residues), 236.5 bits, see alignment E=1.8e-74 PF08345: YscJ_FliF_C" amino acids 257 to 458 (202 residues), 147.4 bits, see alignment E=4.2e-47

Best Hits

KEGG orthology group: K02409, flagellar M-ring protein FliF (inferred from 71% identity to vap:Vapar_4168)

Predicted SEED Role

"Flagellar M-ring protein FliF" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (578 amino acids)

>GFF5750 Flagellar M-ring protein FliF (Variovorax sp. SCN45)
MSTAAPAGELPAAGNGLPPILERLRAQPKLPLIVGGAALVAAAAAFALWSRGPDYKVLYT
NVSDRDGGAIIASLQQMNVPYKFAEGGGAILIAGDKVAETRLKLAAQGLPKAGGVGFELM
DNQKFGTSQFAEQVNYQRGLEGELARSIESIGSIESARVHLALPKPSLFVRDQKKPSASV
VLNLMRGRSIDEGQVSAIVHMVSSSVPDLDAKSVTVVDQRGNLLSSARGTDRGLDVSQLK
YAQEIEQNYIRRIEAILQPIVGANNVRAQVAAEIDFSVVEHTDEKYKPNQDPAQAAVRSQ
QSSESAQHNGAPPGGVPGALTNQPPVNPTAPIVGPRAPNAPGTPPPGTPGAPGTPGAQGA
TTASTAGAATGPSSTRKDTTTNYELDRTIRHVQQAAGGVKRLSVAVVVNNRDATDAAGKV
NSRALTPAELEQIRNLAKEAMGFSQDRGDSLNVVNSAFARDADSGPAPEVPFWRDRENIE
IGKTVGQYLFYALLALFAWLAVLRPLTRRHAPAAPTPVPVAEQEPAVEPDVNAQSAEESL
REREANRKKADMDYAHQIGDKDPKLVAALIQHWMNTDE