Protein Info for GFF575 in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868

Annotation: 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 728 PF22019: GlgB_N" amino acids 7 to 99 (93 residues), 89 bits, see alignment E=3.5e-29 amino acids 113 to 206 (94 residues), 35.2 bits, see alignment E=2.1e-12 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 109 to 723 (615 residues), 1089.6 bits, see alignment E=0 PF02922: CBM_48" amino acids 125 to 207 (83 residues), 91.7 bits, see alignment E=5.8e-30 PF00128: Alpha-amylase" amino acids 273 to 346 (74 residues), 29.7 bits, see alignment E=8.3e-11 PF02806: Alpha-amylase_C" amino acids 628 to 724 (97 residues), 91 bits, see alignment E=1e-29

Best Hits

Swiss-Prot: 100% identical to GLGB_SALTY: 1,4-alpha-glucan branching enzyme GlgB (glgB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 94% identity to ecg:E2348C_3678)

MetaCyc: 93% identical to 1,4-alpha-glucan branching enzyme (Escherichia coli K-12 substr. MG1655)
1,4-alpha-glucan branching enzyme. [EC: 2.4.1.18]

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (728 amino acids)

>GFF575 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18) (Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868)
MSSRIDRDVINALIAGHFADPFSVLGMHQTQAGLEVRALLPDATDVWVIEPKTGRKVGKL
ECLDARGFFCGVLPRRKNFFRYQLAVTWHGQQNLIDDPYRFGPLIQEMDAWLLSEGTHLR
PYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWDGRRHPMRLRKESGIWELFIP
GAHNGQLYKFELLDANGNLRIKADPYAFEAQMRPETASMICGLPEKVTPGEERQKANQFD
APISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYAKWMGFTHLELLPVNEHPFDGSWGY
QPTGLYAPTRRFGTRDDFRYFINAAHAAGLNVILDWVPGHFPSDEFSLAEFDGTHLYEHS
DPREGYHQDWNTLIYNYGRREVSNYLVGNALYWMERFGIDALRVDAVASMIYRDYSRKEG
EWIPNEFGGRENLEAIEFLRNTNRIIGEQVPGAVSMAEESTDFSGVTRPPETGGLGFWYK
WNLGWMHDTLDYMKLDPVYRQYHHDKLTFGMLYNHTENFVLPLSHDEVVHGKKSILDRMP
GDAWQKFANLRAYYGWMWAFPGKKLLFMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQ
RLVRDLNHTYRHHKALHELDFDAYGFEWLVVDDNERSVLIFVRRDKAGNEIIVASNFTPV
PRHDYRFGINQPGRWREILNTDSMHYHGSNTGNGGVVHSDEIESHGRQHSLNLTLPPLAT
IWLMREGE