Protein Info for GFF5741 in Variovorax sp. SCN45

Annotation: Flagellar biosynthesis protein FliO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 167 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 52 to 74 (23 residues), see Phobius details TIGR03500: flagellar biosynthetic protein FliO" amino acids 61 to 127 (67 residues), 81.5 bits, see alignment E=1.7e-27 PF04347: FliO" amino acids 72 to 159 (88 residues), 78.3 bits, see alignment E=1.9e-26

Best Hits

KEGG orthology group: K02418, flagellar protein FliO/FliZ (inferred from 67% identity to vap:Vapar_4177)

Predicted SEED Role

"Flagellar biosynthesis protein FliQ" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (167 amino acids)

>GFF5741 Flagellar biosynthesis protein FliO (Variovorax sp. SCN45)
MNLRRHLPAWGAAAPALLFLLCARAAQAALPTVPSPTHEAASAAPSAVGAGSLLQAGFGM
AVVVGLIFLCAWLARRFGLQRLAGGQHVKVVSSAMVGQRERVVVIEVADTWVVLGVTASQ
VNTLHTLPAQAASSNNHAASPDGARGPVELFARKLRESLSGKAHTAP