Protein Info for GFF5735 in Variovorax sp. SCN45

Annotation: Flagellar hook-associated protein FlgK

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 654 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details TIGR02492: flagellar hook-associated protein FlgK" amino acids 4 to 361 (358 residues), 266 bits, see alignment E=4.2e-83 PF21158: flgK_1st_1" amino acids 337 to 419 (83 residues), 50.8 bits, see alignment E=2.1e-17 PF21159: FlgK_2nd" amino acids 442 to 543 (102 residues), 56.6 bits, see alignment E=5.1e-19 PF06429: Flg_bbr_C" amino acids 595 to 650 (56 residues), 32.2 bits, see alignment 1.4e-11

Best Hits

KEGG orthology group: K02396, flagellar hook-associated protein 1 FlgK (inferred from 71% identity to vap:Vapar_4182)

Predicted SEED Role

"Flagellar hook-associated protein FlgK" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (654 amino acids)

>GFF5735 Flagellar hook-associated protein FlgK (Variovorax sp. SCN45)
MSGSMFYTGLSGLGVARAALMTTAHNTANVYTAGYSRQVAEIATGGAISSGSGYIGTGAN
VTTVSRSYDRYLTAQLASAQSNSVALATNGTQINRIDTLLADKTSGISALMQNFFTSIQG
VANTPADPAARQQMISSAQALASKFRSTDQYLSDLNTSVNEQIDGSVEQINVYATKIASL
NQQISQATAMASGQPPNDLLDQRDLLVSQLSQVVDVKVLQQDNGKYNVFIATGQSLVVGD
YASQMVSVNSAADPTRKVVALKGFGGNTAELDDSTFKGGVLGGLMTFRSETLIPTQNSIG
RLAMSVSDAVNAQHKLGVDLNGALGQNFFNQAVPGVMSNAKNTGNLEFGATLTDASKLTT
SDYDVSVTNVAGTLTYTVTRLSDKTSMGSFTTFPITFDGVSLNAASGTAQAGDSFLVQPT
RTGARDLDVLVRDPAKVAAASPLVTGNTAGNKGNAALSAATVDASYPGTPLAGPVTLSFD
AATGTLSGFPATSAVTVTLANGTSTTYAAGAAVPYTAGAKMSFDGVTVSMTGAPSQGDTF
TIKNNTSGVSDGSNALLLGALQRKTTMANGTATFNGAYSQLVSEVGNRAMAVKVAAATQD
SVTGQIKASQQSVSGVVQDEETANLLMFQQMYQANAKVIQTASTMFDAILGIRS