Protein Info for GFF5730 in Variovorax sp. SCN45

Annotation: Positive regulator of CheA protein activity (CheW)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 173 PF01584: CheW" amino acids 26 to 162 (137 residues), 136 bits, see alignment E=3.5e-44

Best Hits

Swiss-Prot: 65% identical to CHEW_SALTY: Chemotaxis protein CheW (cheW) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K03408, purine-binding chemotaxis protein CheW (inferred from 87% identity to vap:Vapar_4190)

Predicted SEED Role

"Positive regulator of CheA protein activity (CheW)" in subsystem Bacterial Chemotaxis or Two-component regulatory systems in Campylobacter

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (173 amino acids)

>GFF5730 Positive regulator of CheA protein activity (CheW) (Variovorax sp. SCN45)
MLTKAKDPLQQAASAPSPSTGGSRLEVVTFKLGEEEYGIDIQKVQELRGYDAVTRIANAP
EYIKGVVNLRGIIVPIIDMRIKFKLGDPTYDQFTVVIVLNIGGRVVGMVVDSVSDVITLS
AEQIKPAPEMGSVLDADYLIGLGTLDDRMLILVDIDRLMSSQEMGLIEAAATA