Protein Info for GFF5722 in Variovorax sp. SCN45

Annotation: Flagellar P-ring protein FlgI

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 402 transmembrane" amino acids 39 to 58 (20 residues), see Phobius details PF02119: FlgI" amino acids 60 to 401 (342 residues), 463.1 bits, see alignment E=2.6e-143

Best Hits

Swiss-Prot: 72% identical to FLGI_CUPNJ: Flagellar P-ring protein (flgI) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K02394, flagellar P-ring protein precursor FlgI (inferred from 85% identity to vap:Vapar_4192)

Predicted SEED Role

"Flagellar P-ring protein FlgI" in subsystem Flagellum

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (402 amino acids)

>GFF5722 Flagellar P-ring protein FlgI (Variovorax sp. SCN45)
MKNPQASRTSCRFSFPLAGGDACGPAKPVPRYLTNLRARWRIAAAAVFASLLFVPAHAER
LKELASIQGVRDNPLIGYGLMVGLDGTGDQTMQTPFTTQSLNNMLQQLGITIPQGVNMQL
KNVAAVMVTATLPSFARPGQNIDVTVSSMGNAKSLRGGTLLMTPLKGVDGATYAVAQGNM
VVGGAGASANGSKVQVNQLSSGRIPGGALVERTVEAPVGGEGTFTLELNRSDFGTTQRAV
DAINRQFGPGSAEAIDARVIRVRAPEPQQRVGFLARLEDLEVTPTQAGARVVVNARTGSV
VMNQAVRVKDCAIAHGNLSVVINTEPVISQPGALSGGTTVAAQTSQISVNQGGGAMQMVR
GGASLSDVVKGLNALGANPQDLVSILQAMKSAGALSAELEII