Protein Info for GFF5719 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Allophanate hydrolase (EC 3.5.1.54)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02713: allophanate hydrolase
  • amino acids 41 to 608 (568 residues), 767.8 bits, E=3.2e-235
• PF01425: Amidase
  • amino acids 52 to 442 (391 residues), 260.9 bits, E=1.3e-81

Best Hits

KEGG orthology group: K01457, allophanate hydrolase [EC: 3.5.1.54] (inferred from 60% identity to dar:Daro_0075)

Predicted SEED Role

"Allophanate hydrolase (EC 3.5.1.54)" in subsystem Urea decomposition (EC 3.5.1.54)

MetaCyc Pathways

• L-citrulline degradation (3/3 steps found)
• cyanate degradation (3/3 steps found)
• urea degradation I (3/3 steps found)
• cyanuric acid degradation II (4/5 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• cyanuric acid degradation I (3/5 steps found)
• superpathway of allantoin degradation in yeast (3/6 steps found)
• uracil degradation III (2/5 steps found)
• superpathway of atrazine degradation (4/8 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Atrazine degradation
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.54

Use Curated BLAST to search for 3.5.1.54

Protein Sequence (613 amino acids)

>GFF5719 Allophanate hydrolase (EC 3.5.1.54) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSLTQLSFDLASLQAAYREGTANVREVIAEALRRSAADTHHAYIRLLSAAEVEPYLARLD
GVDPASLPLFGVPFAIKDNIDLAHIPTTAACPEFAHTPADSAFVVRQLLAAGAVPVGKTN
LDQFATGLNGTRSPYGACRNAHNPAFISGGSSSGSGVVVAKGYVSFALGTDTAGSGRVPA
AFNGLIGLKPTLGLLSASGVLPACRSVETPSILALTVADAQAVLGVAAAFDDTDAYSRPA
QPLGVDFSAGPFRFGVPRDEDLDFFGNGAAAALFADAVKRLESLGGTAVTIDLTPFLDVA
KLLYEGPWVAERYVAIQGFIDTQPEAVFPPVRSIIEGGRGKTASEAFAGAYRLKALRRVC
DRVWNEVDCLLTPTAGTIYRIEEMQADPIRLNANLGRYTNFVNLLDQCAVAVPAGVLASG
DAKGLPWGVTLGAPAHKDLALLRLADRFHRALGGTLGATGTDVSATPASAGLPGGEVGAA
GAGTVRVAVCGAHLAGLPLNHQLTSRGARLLCSPPSAAEYQLHALAGGPVQRPGMVRVAE
GGAAIELEVWEVPTALFGSFVAGIPAPLGIGKVKLADGSEVPGFICEGSGIAGATDITHF
GGWRAWLAAGAPR