Protein Info for GFF5718 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Urea carboxylase (EC 6.3.4.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1100 1175 PF00289: Biotin_carb_N" amino acids 1 to 110 (110 residues), 154.6 bits, see alignment E=5.7e-49 PF02786: CPSase_L_D2" amino acids 116 to 319 (204 residues), 215.8 bits, see alignment E=2.4e-67 PF07478: Dala_Dala_lig_C" amino acids 144 to 291 (148 residues), 43.4 bits, see alignment E=1.3e-14 PF02785: Biotin_carb_C" amino acids 336 to 440 (105 residues), 112.7 bits, see alignment E=4.3e-36 TIGR00724: biotin-dependent carboxylase uncharacterized domain" amino acids 448 to 745 (298 residues), 298.2 bits, see alignment E=6.3e-93 PF02626: CT_A_B" amino acids 472 to 744 (273 residues), 333 bits, see alignment E=7.1e-103 PF02682: CT_C_D" amino acids 769 to 987 (219 residues), 135.9 bits, see alignment E=6.7e-43 PF00364: Biotin_lipoyl" amino acids 1104 to 1168 (65 residues), 47.6 bits, see alignment 5.4e-16 PF13533: Biotin_lipoyl_2" amino acids 1104 to 1137 (34 residues), 29.2 bits, see alignment (E = 2.8e-10)

Best Hits

Predicted SEED Role

"Urea carboxylase (EC 6.3.4.6)" in subsystem Urea decomposition (EC 6.3.4.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.4.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1175 amino acids)

>GFF5718 Urea carboxylase (EC 6.3.4.6) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MFSKVLIANRGAIACRIIRTLKTLGVRSVAVYSEADAQARHVREADEAHLLGPAPAAESY
LRADRILQIAKDCGAQAIHPGYGFLSENPAFAEACEAAGIAFIGPAPQQMRAFGLKHTAR
DLAQARGVPLLPGSGLLLDLAQATAEAARIGYPVMLKSTAGGGGIGMRLVWNDTELRDAF
ESVGRLAQANFKDAGLFLEKYVEQARHIEVQVFGDGAGGVIALGERDCSVQRRNQKVIEE
TPAPGLSDAQRTELHATAVRLAQAVNYRSAGTVEFVYDAASGAFYFLEVNTRLQVEHGVT
EQVCGVDLVAWMLQLAAGDLPPLATLQPVPRGASIQVRLYAEDPAKNFQPSAGLLTDVVF
PPRARVDSWVERGTEVPAWYDPMLAKLIVTADTREQALLQLEDALDATRLAGIETNLGYL
RQVVRDPVFREGRQVTRFLATFDFKPRTIDVLEPGVQTSVQDSPGRQGYWAVGVPPSGPM
DTHAHRMANQLVGNASDAAALEITLAGPTLRFNVATVVAVTGAPAKLTLNGQPVAMWQAL
AVPAGATLAVGRCETGVRLALAVAGGLDVPLYLGSRSTFTLGQFGGHAGRHLRTGDVLHL
ADAPASVEAQTMSDYVYAPEYTNHWDIDVLYGPHGAPDFFTPEDIAMFFGTDWKVHHNSS
RTGVRLIGPKPQWARSDGGEAGLHPSNIHDNAYAIGAIDFTGDMPVILGPDGPSLGGFVC
PAVVLHDELWKLGQLRPGDTVHFHRRAGSPASPILDDRAAEGERPRRVVRQAGDRYVLIE
FGPLTLDLELRMRVQALLEGLKRKHLGGVIDLTPGIRSLQVHFDPTRLPREQLLRVIAEV
DDALPPVDEMVVPSRTVVLPLSWDDPQTKLAIEKYMQSVRPDAPWCPSNIEFIRRINGLD
SIEDVFQVVFDARYLVLGLGDVYLGAPVATPLDPRHRLVTTKYNPARTWTPENAVGIGGA
YLCVYGMEGPGGYQFVGRTLQMWNRWRHGTPGTPFEQPWLLRFFDQIRFEPVSEDALKDI
RARFPHGGYALRTEDGTFSLKDYRRFLAENGEAISAFKTRQQAAFEAERERWAAAGQVDY
ASEADVSAAGVEAEIDLPEGGRALATSVPGNVWKVAVSVGQQVQAGDVLLVIESMKMEFN
LLAPANATVHSLMCAQGGSVAAGQNVLVLIDEAAV