Protein Info for GFF5716 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR03425: urea carboxylase-associated protein 2
  • amino acids 32 to 263 (232 residues), 329 bits, E=6.4e-103
• PF09347: DUF1989
  • amino acids 36 to 208 (173 residues), 190.6 bits, E=8.5e-61

Best Hits

KEGG orthology group: K09967, hypothetical protein (inferred from 68% identity to dar:Daro_0072)

Predicted SEED Role

"Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10)" in subsystem Urea decomposition (EC 2.1.2.10)

MetaCyc Pathways

• glycine biosynthesis II (3/3 steps found)
• glycine cleavage (3/3 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Nitrogen metabolism
• One carbon pool by folate

Isozymes

Compare fitness of predicted isozymes for: 2.1.2.10

Use Curated BLAST to search for 2.1.2.10

Protein Sequence (264 amino acids)

>GFF5716 Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNHTLNDTPVDAPAFWQGFPAPALAGVDTHRVMWSEIVPGGSHWGWRLPRGSAIRFVALE
AGANVSLVLYSAQEKLERYNMPDSLKAQHTAHYTRGHVLMSDMGRSMASFTADSLGWHDP
LGALLDSELMQQKYGEKRYETHRNAMHRSGKDGLLIEIGKHGLSKRDLIAPVNLFSKVGV
DAQGRFAFVPDHAKAGDFVELRFDMDVIAAISTAPHGLDPAPAYAPKKVGLVAWRCGAAP
ADDVCRAFRPENARALHNTDLLYL