Protein Info for GFF5698 in Variovorax sp. SCN45

Annotation: 2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16) / 3'-nucleotidase (EC 3.1.3.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 signal peptide" amino acids 1 to 35 (35 residues), see Phobius details PF00149: Metallophos" amino acids 66 to 316 (251 residues), 29.7 bits, see alignment E=8.4e-11 PF02872: 5_nucleotid_C" amino acids 482 to 603 (122 residues), 69 bits, see alignment E=5.2e-23

Best Hits

KEGG orthology group: K01119, 2',3'-cyclic-nucleotide 2'-phosphodiesterase [EC: 3.1.4.16] (inferred from 71% identity to aav:Aave_3782)

Predicted SEED Role

"2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)" in subsystem Purine conversions (EC 3.1.4.16)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.4.16

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (700 amino acids)

>GFF5698 2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16) / 3'-nucleotidase (EC 3.1.3.6) (Variovorax sp. SCN45)
MKNAAAMSLSRLAPMSTCAAVIAAVLTACGGGSGGGTVIPPFTPTPDPAPVAVAKGTRAT
VALLETTDLHANVRSFDYFKLAEDKSYGYERTATLINAARKEFQNTMLFDNGDTIQGTAL
ADYEALVSRVPCTQPLSVYKVMNATGYDAGTLGNHEFNYGLDFLNQVLGGGLDVDGVDGT
KKCAGPTFPMVLANVQSVKSGKPLLAPYTIVTKQFTATTPDGKTVTVPVKVGVIGFTTPG
ILNWDKRFLEGKVRTEGAVEAATKYIPELRAKGADIVVALQHGGLDASPYSPTMESPGLY
LSKVPGIDALMMGHQHGTFPDPGTKPSYTQAGVDNKAGTVNGVPAVMASSWGKALGLIRL
DLVYDGKAWGVDKTKTFVEARSTQSKDSAGKVVYVDADSSVAPLVETQHQATITYVKTPI
GTTDFRMSTYFADVGDPGAIQIVNQAQADYVKNYIAANLPQYASLPVLSVSAPFKSGYEG
GRDYTDVAAGNVAINNAADLYLYPNTVYAVKVTGAEIKAWLEAAAKRFNKINPDLTTDQQ
LISTFPGYNFDMFTTPDVQYEIDVTQAEGSRIKNLTYLSAPIDPAKEFVIATNNYRATSG
ASFIPALDGRATIYPSPDANRDVLIDYIKAKKTITRTANGAARSWRFTQVTTKGNVVFTS
GQGQLSAAQDAGLTNISLLTADDGSGKGRSVYKLELNPAP