Protein Info for GFF5681 in Variovorax sp. SCN45

Annotation: Predicted gluconate TRAP family transporter, DctM subunit

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 646 transmembrane" amino acids 51 to 77 (27 residues), see Phobius details amino acids 89 to 107 (19 residues), see Phobius details amino acids 128 to 149 (22 residues), see Phobius details amino acids 169 to 190 (22 residues), see Phobius details amino acids 201 to 219 (19 residues), see Phobius details amino acids 225 to 258 (34 residues), see Phobius details amino acids 278 to 299 (22 residues), see Phobius details amino acids 303 to 330 (28 residues), see Phobius details amino acids 361 to 384 (24 residues), see Phobius details amino acids 390 to 416 (27 residues), see Phobius details amino acids 437 to 455 (19 residues), see Phobius details amino acids 461 to 481 (21 residues), see Phobius details amino acids 493 to 517 (25 residues), see Phobius details amino acids 538 to 569 (32 residues), see Phobius details amino acids 581 to 605 (25 residues), see Phobius details amino acids 621 to 643 (23 residues), see Phobius details PF04290: DctQ" amino acids 66 to 193 (128 residues), 112 bits, see alignment E=2e-36 PF06808: DctM" amino acids 232 to 641 (410 residues), 329.8 bits, see alignment E=2.4e-102

Best Hits

KEGG orthology group: None (inferred from 87% identity to vap:Vapar_5667)

Predicted SEED Role

"Predicted gluconate TRAP family transporter, DctM subunit" in subsystem D-gluconate and ketogluconates metabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (646 amino acids)

>GFF5681 Predicted gluconate TRAP family transporter, DctM subunit (Variovorax sp. SCN45)
MSAPRPAARPPEPPTGGASGNAPPVSSHGHGDAANEANPASFTSRTEHVLGVLVDGLAAA
LVLADVAVLLAGVVARFVLHQPLIWSDELASMLFIWLAMLGAVVALRRGEHMRMTAMVSK
LPPQRRRLAEAIALYAALAFLAMVAWPAWEYASEERFITTPALDISNAWRAAALPVGVVL
MGMFAVLRLFRDFDWKTSTKAFAVVVTLILAFTLGRAVFEDLGRLNLLVFFVGVVLACVL
CGIPIAFAFGLGTFGYLALTTDTALPVIIGRMDEGMSHLILLAVPLFVFLGLLIEMTGMA
KAMVAFLASLLGHVKGGLSYVLVGAMYLVSGISGSKAADMAAIAPALFPEMKKRGADEGE
LVALLAATGAQTETVPPSLVLITIGSVTGVSIAALFTGGLLPAVILGLMLCVVVFMRNRH
EDLSQVARPPWKQVMRLALVALPALALPFVIRAAVVEGVATATEVSTIGIVYAVVAGLVV
YRQFDWRRLGPMLVATASLSGAILLIIGTATAMAWGLTQSGFSRWLAEAMASLPGGAPMF
LAVSLVTFVVLGSVLEGIPAIVLFGPLLFPIARQLGIHEVHYAMVVVLSMGLGLFAPPLG
VGYYAACAIGRVRPDAGIRPIVGYMLALLVGTIAVAAIPWLSIGFL