Protein Info for GFF5671 in Variovorax sp. SCN45

Annotation: FIG01964566: Predicted membrane protein, hemolysin III homolog

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 228 transmembrane" amino acids 28 to 51 (24 residues), see Phobius details amino acids 57 to 79 (23 residues), see Phobius details amino acids 97 to 116 (20 residues), see Phobius details amino acids 122 to 141 (20 residues), see Phobius details amino acids 148 to 169 (22 residues), see Phobius details amino acids 175 to 196 (22 residues), see Phobius details amino acids 206 to 225 (20 residues), see Phobius details PF03006: HlyIII" amino acids 24 to 219 (196 residues), 131.1 bits, see alignment E=2.6e-42 TIGR01065: channel protein, hemolysin III family" amino acids 25 to 223 (199 residues), 182.7 bits, see alignment E=3e-58

Best Hits

Swiss-Prot: 40% identical to YQFA_SHIFL: UPF0073 inner membrane protein YqfA (yqfA) from Shigella flexneri

KEGG orthology group: K11068, hemolysin III (inferred from 93% identity to vap:Vapar_4053)

Predicted SEED Role

"COG1272: Predicted membrane protein hemolysin III homolog"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (228 amino acids)

>GFF5671 FIG01964566: Predicted membrane protein, hemolysin III homolog (Variovorax sp. SCN45)
MTSRSQTQPSPRASVLAARDQTAMEELFNALSHGLGLALAIAALPILVYSAAQKGQAAGV
VGACLFAGTAIVLYLISTLYHALPMGRAKAWFNRLDHAAIYLFIAGSYMPFLFGVLRGPL
GWTLFGAICAAAALGVGAKLFNRLQHPLWSTGLYVAMGWMALMAAVPLYERMSAAGLGWL
VAGGLFYTAGAVVFLFDNKVRYAHSVWHLFVLAGSTCHFFAVLWHSHG