Protein Info for GFF5662 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K00074, 3-hydroxybutyryl-CoA dehydrogenase [EC: 1.1.1.157] (inferred from 56% identity to rsl:RPSI07_1415)

Predicted SEED Role

"3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation (EC 1.1.1.35, EC 4.2.1.17)

MetaCyc Pathways

oleate β-oxidation (29/35 steps found)
phenylacetate degradation I (aerobic) (8/9 steps found)
adipate degradation (5/5 steps found)
superpathway of phenylethylamine degradation (9/11 steps found)
benzoyl-CoA degradation I (aerobic) (6/7 steps found)
2-methyl-branched fatty acid β-oxidation (11/14 steps found)
superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
benzoyl-CoA biosynthesis (3/3 steps found)
fatty acid salvage (5/6 steps found)
1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
valproate β-oxidation (7/9 steps found)
4-hydroxybenzoate biosynthesis III (plants) (4/5 steps found)
adipate biosynthesis (4/5 steps found)
photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (19/26 steps found)
fatty acid β-oxidation I (generic) (5/7 steps found)
L-isoleucine degradation I (4/6 steps found)
pyruvate fermentation to butanol II (engineered) (4/6 steps found)
superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
(R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (3/5 steps found)
fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
fatty acid β-oxidation IV (unsaturated, even number) (3/5 steps found)
glutaryl-CoA degradation (3/5 steps found)
pyruvate fermentation to hexanol (engineered) (7/11 steps found)
pyruvate fermentation to butanoate (4/7 steps found)
methyl ketone biosynthesis (engineered) (3/6 steps found)
propanoate fermentation to 2-methylbutanoate (3/6 steps found)
glycerol degradation to butanol (10/16 steps found)
L-valine degradation I (4/8 steps found)
(8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
3-hydroxypropanoate/4-hydroxybutanate cycle (11/18 steps found)
fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
3-phenylpropanoate degradation (5/10 steps found)
L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
6-gingerol analog biosynthesis (engineered) (2/6 steps found)
benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
benzoate biosynthesis III (CoA-dependent, non-β-oxidative) (1/5 steps found)
pyruvate fermentation to butanol I (3/8 steps found)
L-glutamate degradation VII (to butanoate) (5/12 steps found)
2-methylpropene degradation (2/8 steps found)
L-tryptophan degradation III (eukaryotic) (7/15 steps found)
methyl tert-butyl ether degradation (2/10 steps found)
superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (7/17 steps found)
superpathway of Clostridium acetobutylicum solventogenic fermentation (4/13 steps found)
benzoate fermentation (to acetate and cyclohexane carboxylate) (6/17 steps found)
crotonate fermentation (to acetate and cyclohexane carboxylate) (5/16 steps found)
(4Z,7Z,10Z,13Z,16Z)-docosapentaenoate biosynthesis (6-desaturase) (2/13 steps found)
docosahexaenoate biosynthesis III (6-desaturase, mammals) (2/14 steps found)
toluene degradation VI (anaerobic) (4/18 steps found)
cholesterol degradation to androstenedione I (cholesterol oxidase) (2/17 steps found)
Spodoptera littoralis pheromone biosynthesis (4/22 steps found)
androstenedione degradation I (aerobic) (6/25 steps found)
platensimycin biosynthesis (6/26 steps found)
cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (3/22 steps found)
superpathway of testosterone and androsterone degradation (6/28 steps found)
androstenedione degradation II (anaerobic) (4/27 steps found)
superpathway of cholesterol degradation I (cholesterol oxidase) (8/42 steps found)
superpathway of cholesterol degradation II (cholesterol dehydrogenase) (9/47 steps found)
superpathway of cholesterol degradation III (oxidase) (5/49 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.157, 1.1.1.35, 4.2.1.17

Use Curated BLAST to search for 1.1.1.157 or 1.1.1.35 or 4.2.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (508 amino acids)

>GFF5662 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / Enoyl CoA hydratase (EC 4.2.1.17) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MTSNNVKPLTLGVVGAGAMGRGIVQLFAQAGHRVRCFDAAPGAAAKAVEFALGMIGRGVE
KGRLSAEAFEAIRASVSPCDDMAGLAGCDVVIEAIVEDLAVKRQLFGALEDVVAETTVLA
SNTSSLTVADIAAACRHPARVAGLHFFNPVPLMKVAEVIAALRTSPQTVQTLSELVSGAG
HRAVVTADQPGFLVNHAGRGLYTEGLRLLEEQAASPADVDDVLREGVGFRMGPFELLDLT
GLDVSSKVMASIYEQFQHEPRFRPSSLVAPRVAAGLFGRKTGEGWYRYDGDARRAAPARA
VPAPRAGVAVWVDPQASGRAELAALLSGAGARLTDDAAQAEVCVVQPWGGDASHAAAALG
LDAARTVAVDPMVPLALRRTLMLTALTSAAARDAAWGLLAADGVPVTVINDSPGFIAQRV
LATIVNIAAQIAQRGIASVADIEDAVKLGLGYPHGPLAWGDRIGGARILQILRTMQAETG
DPRYRPSPWLVRRVALGVPLLTPEAERA