Protein Info for GFF5653 in Variovorax sp. SCN45

Annotation: Acyl-CoA dehydrogenase (EC 1.3.8.1), Mycobacterial subgroup FadE16

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 371 PF02771: Acyl-CoA_dh_N" amino acids 14 to 99 (86 residues), 53.3 bits, see alignment E=6.7e-18 PF02770: Acyl-CoA_dh_M" amino acids 109 to 200 (92 residues), 34.6 bits, see alignment E=3.5e-12 PF00441: Acyl-CoA_dh_1" amino acids 213 to 365 (153 residues), 72.4 bits, see alignment E=9.5e-24 PF08028: Acyl-CoA_dh_2" amino acids 231 to 346 (116 residues), 44.6 bits, see alignment E=3.6e-15

Best Hits

KEGG orthology group: None (inferred from 59% identity to del:DelCs14_2302)

Predicted SEED Role

"Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)" in subsystem Isoleucine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.2

Use Curated BLAST to search for 1.3.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (371 amino acids)

>GFF5653 Acyl-CoA dehydrogenase (EC 1.3.8.1), Mycobacterial subgroup FadE16 (Variovorax sp. SCN45)
MNPLEVLAGIETQTLQPQAQRTDSEGRFPRETVSAMGQAGLLGLVSATAVGGQGLGLKEA
AQVVSRIAQTCPSTAMVVCMHYCATAVLEQFGPEATRRAIAEGKHLSTLAFSEADSRSHF
WAPTGTARADGANVVLDARKSWVTSAFEADSYVWSSKPLAAEGASTLWLVDAKSDGLSQP
GRFDGLGLRGNASTPVTAKDMRIAASARLGEDGKGFDTMMGVVLPWFSVLSAACSTGLME
GALSRAIGHVSQTRHLHLDSSLADLPTIRAYLARARIKTDMAQALLDDTVAAIGAGRADT
MLRVLEVKAAAAETALEVTDIAMRVCGGAAFRKEAGIERLFRDARASSVMAPTSDVLYDF
IGKAITGLPLF