Protein Info for GFF5617 in Variovorax sp. SCN45
Annotation: Coenzyme A ligase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K01912, phenylacetate-CoA ligase [EC: 6.2.1.30] (inferred from 91% identity to vap:Vapar_5012)Predicted SEED Role
"Phenylacetate-coenzyme A ligase (EC 6.2.1.30)" in subsystem Aromatic amino acid interconversions with aryl acids (EC 6.2.1.30)
MetaCyc Pathways
- phenylacetate degradation I (aerobic) (8/9 steps found)
- superpathway of phenylethylamine degradation (9/11 steps found)
- penicillin G and penicillin V biosynthesis (3/4 steps found)
- phenylacetate degradation II (anaerobic) (1/4 steps found)
- L-phenylalanine degradation IV (mammalian, via side chain) (4/9 steps found)
- anaerobic aromatic compound degradation (Thauera aromatica) (6/27 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.30
Use Curated BLAST to search for 6.2.1.30
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (416 amino acids)
>GFF5617 Coenzyme A ligase (Variovorax sp. SCN45) MTDHYDKLEIRDPAERERDLLAALPRQIAQAQTTAPAFAKILDGVKPADITTREALAKLP VTRKSELLELQKARRADDPFGGFASIVRGPRMPRVFASPGTIYEPEGEARDYWRTARALH AAGFRGGELIHNSFSYHMTPAGSIMESGARALGCTVFAGGTGQTEQQLEAMVDLKPEGYA GTPSFLKILLEKAEEKGLKLPSLTKALVSGEAFPPSLHDWIAAHGVKGTQCYATADLGLI AYETSARQGLVIDEGVLVEIVRPGTGDPVPDGEVGEIVVTSFNLDYPLVRFGTGDLSAVL AGTCPTGRTNKRIKGWLGRADQTTKVRGMFVHPSQVAEILRRFPEAQRARLVVSGEMGDD RMALQLECAGAVPEGLQARVGDAIREVTKLRGEVVVVGAGELPNDGKVIEDARSYK