Protein Info for GFF5617 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Butyryl-CoA dehydrogenase (EC 1.3.99.2)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 38% identical to ACADL_HUMAN: Long-chain specific acyl-CoA dehydrogenase, mitochondrial (ACADL) from Homo sapiens
KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 53% identity to gpb:HDN1F_19800)MetaCyc: 38% identical to long-chain-acyl-CoA dehydrogenase monomer (Homo sapiens)
LONG-CHAIN-ACYL-COA-DEHYDROGENASE-RXN [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; RXN-21775 [EC: 1.3.8.8, 1.3.8.7]
Predicted SEED Role
"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)
MetaCyc Pathways
- oleate β-oxidation (29/35 steps found)
- adipate degradation (5/5 steps found)
- 2-methyl-branched fatty acid β-oxidation (11/14 steps found)
- fatty acid salvage (5/6 steps found)
- 1-butanol autotrophic biosynthesis (engineered) (20/27 steps found)
- pyruvate fermentation to butanol II (engineered) (4/6 steps found)
- pyruvate fermentation to hexanol (engineered) (7/11 steps found)
- (8E,10E)-dodeca-8,10-dienol biosynthesis (6/11 steps found)
KEGG Metabolic Maps
- Butanoate metabolism
- Fatty acid metabolism
- Propanoate metabolism
- Valine, leucine and isoleucine degradation
- beta-Alanine metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.2, 1.3.99.3
Use Curated BLAST to search for 1.3.8.7 or 1.3.8.8 or 1.3.99.2 or 1.3.99.3
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (386 amino acids)
>GFF5617 Butyryl-CoA dehydrogenase (EC 1.3.99.2) (Hydrogenophaga sp. GW460-11-11-14-LB1) MTLPYFSEPDIVDEDLRMVREQLRRYVDEVIVPNGDAWEASGEIPRHVFTDLGAMGFLGM RHPVEYGGGGLGALSSLVLGEELSRSTYGGVASALTVHSDMSLSHIAHRGTHEQKERYLP AACAGEKVGVICVTEAHAGSDVAGLKTRAERTDSGWVINGSKMFITNAVFGDIYIVAART DPHAKGSKGLSLFIVEKGNPGLKIARKLDKHGWRSSDTAELYLEDLHVPHDALLGEEGKG FYYIMSTFQNERLVIGALSSGTASRAIELTVEYVKQRQAFGKSLWDQQVVRNKLAWMASK TAAVRSLTYQCAQMIDEGKDIVREVSMLKCFAAETLQEVVHTCLQLHGGTGYMIGTPVER MARDARILTIGGGATEVMLEEVAKRM