Protein Info for GFF561 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 604 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details transmembrane" amino acids 421 to 439 (19 residues), see Phobius details PF21799: MurD-like_N" amino acids 5 to 97 (93 residues), 52.7 bits, see alignment E=6.3e-18 PF08245: Mur_ligase_M" amino acids 260 to 331 (72 residues), 39.2 bits, see alignment E=1.1e-13 TIGR01087: UDP-N-acetylmuramoylalanine--D-glutamate ligase" amino acids 260 to 590 (331 residues), 225.3 bits, see alignment E=8e-71 PF02875: Mur_ligase_C" amino acids 450 to 569 (120 residues), 47.8 bits, see alignment E=4e-16

Best Hits

Predicted SEED Role

"UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (604 amino acids)

>GFF561 UDP-N-acetylmuramoylalanine--D-glutamate ligase (EC 6.3.2.9) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MNEQAVLILGLGASGLAMARWCSRQGARVTVADTRETPPQLAVLRAECPDASFVAGPFDD
ALLARAEWNLIARSPGLAPEVLQGVFGWAKERNVAVVGELDLFARALRELSQRERLPYKP
KVLAITGTNGKTTVTSLTGLLLQRAGLNVAVAGNIGPALLDVLSAALDTEAVAQAADDER
AAAEAAQAQAEAEAQAANAVQAPPVVVDEAPAVEGDAEADPVAEEVEAIETPASDEAEDD
GPTQLVPPPVEPPQPAHLPQAWVLELSSFQLDGTAGGDWDSVPTAATVLNLTEDHLDWHG
SMAAYALAKKAVFGAQALMLLNRDDPLVMGMQPGLVTVKVGNRNRQQSARPWASFGLDAP
TRAGDWGIETVNGMTWLVRALALDETRKRGRGAEEDEETYFQRLMPVDALRIRGRHNAAN
ALAALALASSTGAALGPMLHGLREYRGEPHRVEPVAIIDEVEYFDDSKGTNVGATLAAVS
GLGVERRLVVILGGDGKGQDFTPLAEPLARFARAVVYIGRDAARIREAVGAALAQANVPQ
LDSATLPEAVALCAQQARPGDAVLMSPACASLDMFDNYMHRARVFVDAVHALAAEQGLSL
EGGL