Protein Info for GFF5589 in Variovorax sp. SCN45

Annotation: Methionine aminopeptidase (EC 3.4.11.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00500: methionine aminopeptidase, type I
  • amino acids 8 to 252 (245 residues), 307.7 bits, E=3e-96
• PF00557: Peptidase_M24
  • amino acids 18 to 245 (228 residues), 183.1 bits, E=3e-58

Best Hits

Swiss-Prot: 56% identical to MAP1_SHIFL: Methionine aminopeptidase (map) from Shigella flexneri

KEGG orthology group: K01265, methionyl aminopeptidase [EC: 3.4.11.18] (inferred from 81% identity to vap:Vapar_5754)

MetaCyc: 56% identical to methionine aminopeptidase (Escherichia coli K-12 substr. MG1655)
Methionyl aminopeptidase. [EC: 3.4.11.18]

Predicted SEED Role

"Methionine aminopeptidase (EC 3.4.11.18)" (EC 3.4.11.18)

MetaCyc Pathways

• Arg/N-end rule pathway (eukaryotic) (8/14 steps found)
• Ac/N-end rule pathway (6/21 steps found)

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.18

Use Curated BLAST to search for 3.4.11.18

Protein Sequence (265 amino acids)

>GFF5589 Methionine aminopeptidase (EC 3.4.11.18) (Variovorax sp. SCN45)
MRIAAAPLKSPEEIAMSRAAGRLAAEVLAMIGEHVKPGVTTEELDRICHDHIVDVQGVVP
ANVGYQGYTKTVCTSVNHVVCHGVPSAQKTLKKGDIINIDVAVNKDGWFGDCSRMYFVGE
PSPLARRLVDTTYEAMRAGILQVRPGATLGDVGHAIQSVAHRERFSVVREYCGHGIGQIY
HDDPQVLHYGQPGQGLRLEAGMVFTIEPMINAGRRETRELPDGWTVVTRDHSLSAQWEHM
VAVTEDGFEVLTPWPEGTGRYPAIA