Protein Info for GFF5576 in Variovorax sp. SCN45

Annotation: Acyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 397 signal peptide" amino acids 1 to 28 (28 residues), see Phobius details PF02771: Acyl-CoA_dh_N" amino acids 35 to 128 (94 residues), 39.8 bits, see alignment E=1.1e-13 PF02770: Acyl-CoA_dh_M" amino acids 133 to 226 (94 residues), 80.4 bits, see alignment E=1.8e-26 PF00441: Acyl-CoA_dh_1" amino acids 238 to 395 (158 residues), 143.1 bits, see alignment E=1.5e-45 PF08028: Acyl-CoA_dh_2" amino acids 255 to 382 (128 residues), 64.2 bits, see alignment E=3.1e-21

Best Hits

KEGG orthology group: K00249, acyl-CoA dehydrogenase [EC: 1.3.99.3] (inferred from 95% identity to vap:Vapar_5041)

Predicted SEED Role

"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.2, 1.3.99.3

Use Curated BLAST to search for 1.3.99.2 or 1.3.99.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (397 amino acids)

>GFF5576 Acyl-CoA dehydrogenase (Variovorax sp. SCN45)
MMTKAPAPPSTAHLALPFFDDAHRALADGLLPWAAAQEVDERDDRAACREWVRRLGDGGW
LRYAVPGSAGGALERLDSRALVLLRETLGYHSPLADFAFAMQGLGSGAITLAGTPEQQSR
YLTAVGKGDKIAAFALSEPDAGSDVAAMAMRAEATSDGWRLNGEKTWISNGGIADFYCVF
AKTDPTGGTRGITVFIVDATTPGLDTSAHIDVMAPHPLATLRFDNCVVPRTSQLGELNGG
FKLAMRTLDIFRASVAAAALGMGRRALAEAVAHSKKRRMFGQTLADFQLTQAKLGEMAAL
IDSAALLTYRAAWMRDDAERRGAPAVGDVSAAAAMAKMCATENASRVIDMALQMHGGLGV
KVGTKIESLYRDIRSLRIYEGATEVQQLIIGKSVLRG