Protein Info for GFF5547 in Variovorax sp. SCN45

Annotation: Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54) / Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02682: CT_C_D
  • amino acids 4 to 193 (190 residues), 216 bits, E=4.3e-68
• TIGR00724: biotin-dependent carboxylase uncharacterized domain
  • amino acids 231 to 522 (292 residues), 248.9 bits, E=3.2e-78
• PF02626: CT_A_B
  • amino acids 255 to 521 (267 residues), 285.7 bits, E=4e-89

Best Hits

KEGG orthology group: None (inferred from 81% identity to vpe:Varpa_0700)

Predicted SEED Role

"Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54) / Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54)" (EC 3.5.1.54)

MetaCyc Pathways

• uracil degradation III (5/5 steps found)
• L-citrulline degradation (3/3 steps found)
• cyanate degradation (3/3 steps found)
• urea degradation I (3/3 steps found)
• L-arginine degradation V (arginine deiminase pathway) (3/4 steps found)
• cyanuric acid degradation II (3/5 steps found)
• superpathway of allantoin degradation in yeast (3/6 steps found)
• cyanuric acid degradation I (2/5 steps found)
• superpathway of atrazine degradation (3/8 steps found)
• allantoin degradation IV (anaerobic) (2/9 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Atrazine degradation
• Urea cycle and metabolism of amino groups

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.54

Use Curated BLAST to search for 3.5.1.54

Protein Sequence (530 amino acids)

>GFF5547 Allophanate hydrolase 2 subunit 1 (EC 3.5.1.54) / Allophanate hydrolase 2 subunit 2 (EC 3.5.1.54) (Variovorax sp. SCN45)
MSMRCLSVNLNAVLVELDDLQQTLALLASLRAQPIDGIEELVPAARTLLITFRPAALSAA
ELARRLRLRNLDARETRVDKRIEIPVRYDGEDLAEVAGLLGIAPDEVVRRHTGSDYTVAF
TGFAPGFAYLSGGHPSLNVPRRQVPRTRIPAGAVGLAGSFSGVYPQASPGGWQIIGVTDM
PMWDLSRATPALLQPGDTVRFVDVTALPAASPVPASQAPSAFAATAPGDCALEIRAAGVQ
ALLQDRGRPGQARQGVSVSGAMDQRALQAANRLVGNATDTACIELAYGGFQLVCRGEAVV
AVTGADGPVTLTRADGVSWPLPRYRPCALADGDMLSLGEPVAGIRSYVAVRGGFAVAPVL
GSLSSDTLARIGPPALAAGDVLPVHAVRVGAVVGVPETPPSNLPTMQEEIVLDIVPGPRA
DWFTPEAIAQLCGQPWLVTPQSNRVGLRLAGDRPMERANLAELPSEGTARGSIQVPPSGQ
PVLFLADHPLTGGYPVIASVATHHLDRAGQVPVNARVRFQPVAGLGPVEH