Protein Info for GFF5538 in Variovorax sp. SCN45

Annotation: ATP-dependent DNA helicase RecG (EC 3.6.4.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 710 PF17191: RecG_wedge" amino acids 34 to 178 (145 residues), 44 bits, see alignment E=5.4e-15 TIGR00643: ATP-dependent DNA helicase RecG" amino acids 47 to 681 (635 residues), 671.6 bits, see alignment E=7e-206 PF01336: tRNA_anti-codon" amino acids 74 to 144 (71 residues), 34.8 bits, see alignment E=3.9e-12 PF00270: DEAD" amino acids 288 to 448 (161 residues), 76.2 bits, see alignment E=8e-25 PF04851: ResIII" amino acids 300 to 443 (144 residues), 26.2 bits, see alignment E=2.1e-09 PF00271: Helicase_C" amino acids 488 to 600 (113 residues), 64.4 bits, see alignment E=3.2e-21 PF19833: RecG_dom3_C" amino acids 633 to 694 (62 residues), 38.4 bits, see alignment 3.3e-13

Best Hits

KEGG orthology group: K03655, ATP-dependent DNA helicase RecG [EC: 3.6.4.12] (inferred from 95% identity to vpe:Varpa_5786)

Predicted SEED Role

"ATP-dependent DNA helicase RecG (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (710 amino acids)

>GFF5538 ATP-dependent DNA helicase RecG (EC 3.6.4.12) (Variovorax sp. SCN45)
VPPKKPIPPPAADAAATAAAPPQPPGAGLSQVQRALRKLGLVRDIDFALYLPMRYEDETR
IVRLADTRDGDLAQVEGVVTECEVVYRPRRQLIATIDDGSDTCQLRFFNFYPSQQKQLAV
GARVRVRGEMRGGFVGRQIMHPTVKAAGTALPNALTPVYSTVAGLAQPVLRREVRSGLAR
AVLDETVPVQIGFRGAWDLRASLSFLHYPTPDVAMATLEDHSHPAWQRIKAEELLAQQLS
QLQARLERAAQRAPVLPSPAEPLASSLHAQLLAVLPFGLTGAQQRVGEEITRDLCREIPM
HRLLQGDVGSGKTVVAALAAARAIDAGFQCALMAPTEILAAQHFGKLVGWLDPLLAERGL
RVAWLTGSQKKKERDEMSAAVESGEAALVIGTHAVISEKVRFKNLALAIIDEQHRFGVAQ
RLALRGKAVGELEPHLLMMSATPIPRTLAMSYYADLDVSTLDELPPGRTPIVTKLVADHR
RDEVIDRIHAQIAQGRQVYWVCPLIEESEAVDLRNATETRDELTGTLGEPIQVGLLHSRM
PTAEKQAVMAAFTANEIQVLVSTTVIEVGVDVPNASLMVIEHAERFGLSQLHQLRGRVGR
GAAASACVLLYAPGDSGRVGEAARARLKAMAETGDGFEIARRDLEIRGPGEFLGARQSGA
PLLRFADLTTDTLLLDWARELAPVMLQKHPDLAQRHIDRWLGTKAEYLKA