Protein Info for GFF553 in Sphingobium sp. HT1-2

Annotation: 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (EC 3.7.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 273 PF00561: Abhydrolase_1" amino acids 24 to 257 (234 residues), 93 bits, see alignment E=3.8e-30 PF12146: Hydrolase_4" amino acids 25 to 236 (212 residues), 32.6 bits, see alignment E=7.8e-12 PF12697: Abhydrolase_6" amino acids 26 to 261 (236 residues), 79.5 bits, see alignment E=9.3e-26

Best Hits

KEGG orthology group: None (inferred from 68% identity to ttu:TERTU_1260)

Predicted SEED Role

No annotation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (273 amino acids)

>GFF553 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (EC 3.7.1.-) (Sphingobium sp. HT1-2)
MVFTSHFVDAGGVRTHWLEAGQGPVLVLMHGGGAGADSWGNWKDCIPIFARHFRVIAPDM
IGFGKTDKPSPETYVYDQPGRNRHLADFLDVLDLKGVHIVGNSMGGATAIGVALDRPDLL
ARIVLMGSAGLPIPPKPSPHLLHNLQYDFTREGMRRVIGGLTAPGFQPSDEMVEYRFQLL
EDDAARAALNAINGETRKGTLNYDEERLKTIEHPVLVVNGKDDGVSIMPRAYRFLDLFPN
SWGYIVPHCGHWAMIEATEDFCGAVNRFLGVTP