Protein Info for GFF5513 in Variovorax sp. SCN45

Annotation: N5-carboxyaminoimidazole ribonucleotide synthase (EC 6.3.4.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF22660: RS_preATP-grasp-like
  • amino acids 12 to 108 (97 residues), 114.3 bits, E=4.3e-37
• TIGR01161: phosphoribosylaminoimidazole carboxylase, ATPase subunit
  • amino acids 13 to 374 (362 residues), 369.9 bits, E=8.2e-115
• PF02222: ATP-grasp
  • amino acids 116 to 292 (177 residues), 173.9 bits, E=4e-55
• PF17769: PurK_C
  • amino acids 313 to 376 (64 residues), 66.9 bits, E=1.5e-22

Best Hits

KEGG orthology group: K01589, 5-(carboxyamino)imidazole ribonucleotide synthase [EC: 6.3.4.18] (inferred from 92% identity to vpe:Varpa_5810)

Predicted SEED Role

"Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21)" in subsystem De Novo Purine Biosynthesis (EC 4.1.1.21)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• inosine-5'-phosphate biosynthesis I (6/6 steps found)
• inosine-5'-phosphate biosynthesis II (5/5 steps found)
• inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.21

Use Curated BLAST to search for 4.1.1.21 or 6.3.4.18

Protein Sequence (396 amino acids)

>GFF5513 N5-carboxyaminoimidazole ribonucleotide synthase (EC 6.3.4.18) (Variovorax sp. SCN45)
VSNGDLPILPGATLGVLGGGQLGRMFAHAAQRMGYFTAVLDPDTDSPAGRVSHHHIHTDY
DDVDGLARLAGLADAVTTEFENVPAPSLEHLAVARPVSPGAPAIAIAQDRIAEKAHFTRC
GVPCAPYAVIETAAQLAAVDDNLLPGILKTARLGYDGKGQQRVKTRAELVDAWQATGCVP
CVLEKMLPLEFECSVILARGRDGQVVHFPPQRNLHRDGILAVTEVHPQNMPKTVAQGAVN
SAKSIANGLGYVGVLCVEFFALADGSLVVNEMAPRPHNSGHYTMEACDVSQFELQVRTLA
GLPLAAPRQHSPAIMLNLLGDLWFPAGADKPASPRWGEVLALPGVHLHLYGKMEPRRGRK
MGHLTLTGATLEAVRATASQAALLLGLPAVTTTDLA