Protein Info for GFF5509 in Variovorax sp. SCN45

Annotation: Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 82% identical to PUR7_POLNA: Phosphoribosylaminoimidazole-succinocarboxamide synthase (purC) from Polaromonas naphthalenivorans (strain CJ2)

KEGG orthology group: K01923, phosphoribosylaminoimidazole-succinocarboxamide synthase [EC: 6.3.2.6] (inferred from 97% identity to vap:Vapar_5096)

Predicted SEED Role

"Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6)" in subsystem De Novo Purine Biosynthesis (EC 6.3.2.6)

MetaCyc Pathways

superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
inosine-5'-phosphate biosynthesis I (6/6 steps found)
inosine-5'-phosphate biosynthesis II (5/5 steps found)
inosine-5'-phosphate biosynthesis III (5/6 steps found)

KEGG Metabolic Maps

Purine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (301 amino acids)

>GFF5509 Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC 6.3.2.6) (Variovorax sp. SCN45)
MTTVHTSSIQSLPLLARGKVRDNYAVGEDRILMVASDRLSAFDVIMGEPIPGKGEILTQM
ALWWFERLGQLCPNHLTGDAPESVVTADEVPQVTGRSMLVKRLKPIPVEAVVRGYLAGSG
WKEYQESRSVCGVPLPEGLTNASKLPRPIFTPAAKAAAGEHDENISYERVVEIVGPKLAQ
QIRETSIAIYETAAQIALAKGMIIADTKFEFGLDEAGTLVLMDEVLTPDSSRYWPVEGYQ
AALAAGANPPSYDKQFVRDWLEATKINGKPWDKTPPAPRLPAEVIEKTAAKYREALERLT
G