Protein Info for GFF5487 in Variovorax sp. SCN45

Annotation: Thymidine phosphorylase (EC 2.4.2.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 799 PF07793: DUF1631" amino acids 6 to 790 (785 residues), 451.4 bits, see alignment E=3.5e-139

Best Hits

Predicted SEED Role

"Thymidine phosphorylase (EC 2.4.2.4)" in subsystem Deoxyribose and Deoxynucleoside Catabolism (EC 2.4.2.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.4

Use Curated BLAST to search for 2.4.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (799 amino acids)

>GFF5487 Thymidine phosphorylase (EC 2.4.2.4) (Variovorax sp. SCN45)
MSIARSASSLQLARETRERFVTATEGAIVPLARAIRDRLTTLASEAGNARAMQEHRDDFV
AFQGQGTRWVSLAQTGWRKALASSGGGAAATSSALRLELIGDEEVESNILSSRLAQVIHD
KASFELSDLRLRIQHLEGTTELDARDVLRPETLAKLLVEQWLGAGLSRGMWTRVQDTVHQ
ALVDVIVKAYKDANVFLVANGVMQEIDLKSFVRRTGSGGGGPGGTATSGGPMARDSSMSG
GPHSAQRPGFGFEGSVPSAAAPPATTGGSPLMIARQRAQTALLSLKRFVTARIGGDTGGG
GAGHYQAANPDGRATDVGAGGGGGVGGGPTVPRVFSRTFAGAIADAEVAYRMAATQYMDA
PGEQATLMQQTAVDLRKRTAELKKRAPTTADKATVEIVALMFQAILAEERIPFSARVWFA
RLQMPVLRVAIAEPEFFGTLQHPARMLIDRMGSCVMGFDAAAITGSALEGEIRRVVQVIE
QYPETGQRVFKLVFDEFVAFLNRYLTQSDTTQRVMSVAQQVEQKETMAIQYTIELRKMLN
DMPVRDEIREFLFKIWAEVLAIAALRYGAQGEQTVMLKRVASELVWAASAKPNRTDRARV
IQDLPQLLQRLRQGMALLGIVDEPQEAHIKIIGATLSDAFMSKTEAIPQAKIEAMAERLT
HLEDFVTDVGGASDLPLDAHSIELLLGVDAASIEVIPDAAGAKVAEDMLAWAHELQVGNW
FMLDHNDRVSQVQFVWRSDRKQLHLFASADGRSFLIQAGRLAMYLQAGLLVPAEEETLTV
RATREALAKLDANPERLLN