Protein Info for GFF5486 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: NADP-specific glutamate dehydrogenase (EC 1.4.1.4)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02812: ELFV_dehydrog_N
  • amino acids 56 to 183 (128 residues), 170.2 bits, E=1.6e-54
• PF00208: ELFV_dehydrog
  • amino acids 201 to 445 (245 residues), 306.5 bits, E=1.6e-95

Best Hits

Swiss-Prot: 72% identical to DHE4_ECOLI: NADP-specific glutamate dehydrogenase (gdhA) from Escherichia coli (strain K12)

KEGG orthology group: K00262, glutamate dehydrogenase (NADP+) [EC: 1.4.1.4] (inferred from 85% identity to har:HEAR0384)

MetaCyc: 72% identical to glutamate dehydrogenase (Escherichia coli K-12 substr. MG1655)
Glutamate dehydrogenase (NADP(+)). [EC: 1.4.1.4]

Predicted SEED Role

"NADP-specific glutamate dehydrogenase (EC 1.4.1.4)" in subsystem Arginine and Ornithine Degradation or Glutamate dehydrogenases or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis or Proline Synthesis (EC 1.4.1.4)

MetaCyc Pathways

• L-glutamate and L-glutamine biosynthesis (7/7 steps found)
• L-glutamate biosynthesis III (1/1 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.4

Use Curated BLAST to search for 1.4.1.4

Protein Sequence (447 amino acids)

>GFF5486 NADP-specific glutamate dehydrogenase (EC 1.4.1.4) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MKYTSAHHFLEHVAHQNPGQPEFHQAVSEVMESLWPFIAAHPKYAEHGLLDRLVEPERVV
MFRISWVNDHGDVQVNRGYRIQHSSAIGPYKGGMRFHPSVNLSILKFLAFEQTFKNALTT
LPMGGGKGGSDFDPKGKSPGEVMRFCQALVSELFRHVGSDTDVPAGDIGVGGREVGFMAG
MMKKLSNRADCVFTGKGLSFGGSLIRPEATGYGTVYFAEEMLKQRGQSFQGLRVSVSGSG
NVAQYAVEKAMALGAKVVTVSDSSGTVIDEDGFSPAKLAELMEVKNHLYGRVSDYAERTK
TRFEPGMNPWAVPVDVALPCATQNELNAEDAATLIRNGVICVAEGANMPTTMDAVQRFEQ
AGVLYAPGKASNAGGVATSGLEMSQNAMRLSWPRDEVDARLHTIMRGIHEACLQHGRRAD
GTVSYVDGANIAGFVKVADAMLAQGVI