Protein Info for PS417_28040 in Pseudomonas simiae WCS417

Annotation: histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 transmembrane" amino acids 12 to 44 (33 residues), see Phobius details PF11808: PhoR" amino acids 5 to 90 (86 residues), 93.4 bits, see alignment E=2.7e-30 TIGR02966: phosphate regulon sensor kinase PhoR" amino acids 94 to 425 (332 residues), 463.6 bits, see alignment E=3.5e-143 PF00989: PAS" amino acids 101 to 164 (64 residues), 32.4 bits, see alignment E=2.4e-11 PF13188: PAS_8" amino acids 107 to 159 (53 residues), 23.4 bits, see alignment 1.3e-08 PF00512: HisKA" amino acids 205 to 271 (67 residues), 70.2 bits, see alignment E=3.7e-23 PF02518: HATPase_c" amino acids 319 to 428 (110 residues), 92.1 bits, see alignment E=9.3e-30 PF13581: HATPase_c_2" amino acids 321 to 400 (80 residues), 27.8 bits, see alignment E=6.7e-10

Best Hits

Swiss-Prot: 79% identical to PHOR_PSEAE: Phosphate regulon sensor protein PhoR (phoR) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

KEGG orthology group: K07636, two-component system, OmpR family, phosphate regulon sensor histidine kinase PhoR [EC: 2.7.13.3] (inferred from 98% identity to pfs:PFLU6040)

Predicted SEED Role

"Phosphate regulon sensor protein PhoR (SphS) (EC 2.7.13.3)" in subsystem High affinity phosphate transporter and control of PHO regulon or Phosphate metabolism (EC 2.7.13.3)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UB68 at UniProt or InterPro

Protein Sequence (440 amino acids)

>PS417_28040 histidine kinase (Pseudomonas simiae WCS417)
MNQNWHGTLIRHMLLLITGCLLVGLVSGYYGWSLAVGIALYLGWTLKQLLRLHEWLRQHK
PDEAPPDGYGLWGEVFDSIYHLQRRDQRVRGRLQAVIDRVQESTAALKDAVIMLDSDGNL
EWWNRAAETLLGLKTPQDSGQPVTNLVRHPRFKEYFEQENYEEALEIPSPTNDRVRIQLY
LTRYGNNEHLMLVRDVTRIHQLEQMRKDFVANVSHELRTPLTVICGYLETLLDNVDEINP
RWSRALQQMQQQGSRMQTLLNDLLLLAKLEATDYPSDNHPVAVQSLLQTIKNDALALSGQ
RGQQITLEADPNVLLKGSEGELRSAFSNLVFNAVKYTPDKGNIRIRWWTDEQGAHLSVQD
SGIGIDAKHLPRLTERFYRVDSSRNSNTGGTGLGLAIVKHVLLRHRARLEISSVLGHGST
FTCHFPPTQMTRSRGVTADE