Protein Info for GFF5476 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF11741: AMIN
  • amino acids 33 to 137 (105 residues), 63.1 bits, E=2.3e-21
• PF01520: Amidase_3
  • amino acids 251 to 469 (219 residues), 164.7 bits, E=2.2e-52

Predicted SEED Role

"N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)" (EC 3.5.1.28)

MetaCyc Pathways

• peptidoglycan recycling I (8/14 steps found)
• peptidoglycan recycling II (4/10 steps found)

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.28

Use Curated BLAST to search for 3.5.1.28

Protein Sequence (485 amino acids)

>GFF5476 N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) (Hydrogenophaga sp. GW460-11-11-14-LB1)
MSGALSRRTLLSTGTLVLLLGTQQIARGASVVAVRIWPAEDYTRVTIESDALLQAKQFFV
TDPPRLAVDIEGIDLLPGLRELVGKLKSDDPNIAGIRAAQNAPNVVRLVIDLKKPIKPQV
FNLEPVAAYQHRLVFDLYPLNPPDPLEQLIAQRMKDMDAASERAARLLGLEADKVTKPAT
PADDPLGELIAGRERPKTPVSPNQSPSPTTPVPKPPLGDSDDTAVAGAGPKGSPAKQPPG
GKGKTERLIIIALDPGHGGEDPGAIGPNGTQEKRVVLQIAHKLRERINETRVNGNPMRAF
MTRDKDFFVPLQVRVQKAQRVQADLFISIHADAFLTPRPQGASVYALSTRGATSTAAKWM
ADKENAADLVGGLNVKAADAHVRRALFDMSTTAQINDSLKLGSAMLGHVKKIGKLHKPQV
EQANFAVLRAPDIPSVLVETAFISNPDEEKRLNDPKYQDDLADALVRGIVAYFSKNPPLA
RNRQI