Protein Info for GFF5470 in Hydrogenophaga sp. GW460-11-11-14-LB1
Annotation: Benzoate-CoA ligase (EC 6.2.1.25)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00666, fatty-acyl-CoA synthase [EC: 6.2.1.-] (inferred from 73% identity to bra:BRADO0692)Predicted SEED Role
"Benzoate-CoA ligase (EC 6.2.1.25)" in subsystem Benzoate transport and degradation cluster (EC 6.2.1.25)
MetaCyc Pathways
- benzoate degradation II (aerobic and anaerobic) (1/1 steps found)
- benzoate biosynthesis I (CoA-dependent, β-oxidative) (4/9 steps found)
- benzoate fermentation (to acetate and cyclohexane carboxylate) (6/17 steps found)
- anaerobic aromatic compound degradation (Thauera aromatica) (6/27 steps found)
KEGG Metabolic Maps
- Alkaloid biosynthesis II
- Benzoate degradation via CoA ligation
- Biosynthesis of plant hormones
- Caprolactam degradation
- Ethylbenzene degradation
- Geraniol degradation
- Limonene and pinene degradation
- Propanoate metabolism
- Tyrosine metabolism
- alpha-Linolenic acid metabolism
Isozymes
Compare fitness of predicted isozymes for: 6.2.1.-, 6.2.1.25
Use Curated BLAST to search for 6.2.1.- or 6.2.1.25
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (520 amino acids)
>GFF5470 Benzoate-CoA ligase (EC 6.2.1.25) (Hydrogenophaga sp. GW460-11-11-14-LB1) MPDAVHPVVRRQTIADALRRTAQRLPAKSAVVCGDTQWTYAEFDALVSRLAAGLARIGVA QGDKVAVLARNSHGFAALRFALARRGAVMVPINFMLKADEVAYILRHAGARTLATDSGLA ELARAAAALDTQVQQYLWLPSEDPSEPVAGMHRFDELAACTDPLPEVTLGSYDLAQIVYT SGTESTPKGAMLTHDAVLWQYVSCVINAEIAEDDHALHALPLYHCAQLDVFFGPAVYIGS SNVITAKPVPDNLIALIEKYRITSFFAPPTVWIALLRSPLFDATKLRSLAKGYYGASIMP VEVLKELASRLPSVRLWNLYGQTEIAPLATMLGPDDQLRKPGSCGKAVINVETRVVSDDM RDVAVGEVGEIVHRSPHLMLGYFNDPDRTRAAFDGDWFHSGDLAVIDEEGYITVVDRKKD MIKTGGENVASREVEEMVYRLPAVSEVAVIGLPDPKWVEAVTAVIVVKAGQALTEDEVIA HCNRHMAAFKSPKRVVFSDSLPKNPSGKLLKRELRQRYTG