Protein Info for GFF5443 in Variovorax sp. SCN45

Annotation: Glutamine synthetase adenylyl-L-tyrosine phosphorylase (EC 2.7.7.89) / Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 916 PF03710: GlnE" amino acids 56 to 247 (192 residues), 163.9 bits, see alignment E=4.6e-52 amino acids 532 to 771 (240 residues), 189.7 bits, see alignment E=6.1e-60 PF08335: GlnD_UR_UTase" amino acids 277 to 419 (143 residues), 108.1 bits, see alignment E=3.9e-35 amino acids 814 to 888 (75 residues), 24.8 bits, see alignment E=1.9e-09

Best Hits

KEGG orthology group: K00982, glutamate-ammonia-ligase adenylyltransferase [EC: 2.7.7.42] (inferred from 94% identity to vpe:Varpa_5876)

Predicted SEED Role

"Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42)" in subsystem Ammonia assimilation (EC 2.7.7.42)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.42

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (916 amino acids)

>GFF5443 Glutamine synthetase adenylyl-L-tyrosine phosphorylase (EC 2.7.7.89) / Glutamate-ammonia-ligase adenylyltransferase (EC 2.7.7.42) (Variovorax sp. SCN45)
VNQLPATSTQTAFSSYSRFVQRLRRRYAAELSLLPPGAPRRESMTVAYEALRLRGDSVGD
ALRIVRQLVMERLVTLDCDEQAPLAVVTTAVTELAELALDIACQDACHELDAVHGAPLGP
DGQRAQLWIVGMGKLGARELNVSSDIDLIYLYDLDGETRGDADGRGRLSNQEYFARAVKR
IYALVGDITEHGFVFRVDLALRPNGNSGPSVVSLDALEEYFQVQGREWERFAWMKSRVVA
PRDVVQAGQAQSLRGAVLPFVFRRYLDYSVFDSLRVLHRQIRDQSARRSAGRPERANDVK
LSRGGIREIEFTVQLLQVVRGGQFPELRTRPTLDALQRLARANLMPQETADTLAAAYEFL
RRVEHRIQYLDDQQTHVLPVADDDLRWIAQTLGYADCCAFLAQLDTHREFVAQEFDKLLG
GEKPCNGKCSGKKAAAPAELADLLDDLPQVFAERIRDWCEQPRVLALREETRVRLRQLVQ
RTGVWLKEADGDGPGQTPRHVDAAMRWADWIEPLMRRESYLALLVERPAVQERLLRLLGA
ARWPARYLMQHPGVIDELASDEMLSGRFVAAEFERELEARHASLTRTGEADEERLLNLLR
HAHHAEVFRTLARDVDGRITVEQVADDLSALADTTLRVTARWCWPHVRNRHREVPQFAII
GYGKLGGKELGYGSDLDIVFVYDDSDERAGEVYAAYVRKLINWLTVKTREGDLFEIDTAL
RPNGNSGLLTTSFEAYEKYQLGRGSNTAWTWEHQAMTRARFVLGSADHGAELGARFDQVR
EAVLVAPRDREALKAEIIAMREKLRGARPVKAGRFDVKHSPGGMVDAEFAVQFLVLSSSG
EHPELIPNVGNIALLLRAELAGLLPEGVGRSAARAYRELRRVQHRARLNEEPTQVTPPAL
AAERDAMLALWKAVFG