Protein Info for GFF5440 in Variovorax sp. SCN45

Annotation: RNA polymerase ECF-type sigma factor

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 296 TIGR02957: RNA polymerase sigma-70 factor, TIGR02957 family" amino acids 7 to 289 (283 residues), 362.1 bits, see alignment E=1.8e-112 TIGR02937: RNA polymerase sigma factor, sigma-70 family" amino acids 8 to 163 (156 residues), 64.8 bits, see alignment E=7.4e-22 PF04542: Sigma70_r2" amino acids 8 to 71 (64 residues), 59.8 bits, see alignment E=2.7e-20 PF08281: Sigma70_r4_2" amino acids 110 to 160 (51 residues), 49.5 bits, see alignment 3.9e-17

Best Hits

KEGG orthology group: K03088, RNA polymerase sigma-70 factor, ECF subfamily (inferred from 88% identity to vap:Vapar_5163)

Predicted SEED Role

"Putative RNA polymerase sigma factor"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (296 amino acids)

>GFF5440 RNA polymerase ECF-type sigma factor (Variovorax sp. SCN45)
MDDATLTFNRHRPRLQGIAYRMLGSVAEAEEVVQDAWLRWHEAAKNTLDSAEAWLVTVVT
RLSIDRLRAAKVQREHYIGAWMPEPTLTDASDSPATPEQLLERADNISVAFLAVLERLAP
EARAAFLLREVFDADYDEVARTLGKSEAACRQLVHRAKAQVQEGRPRFSVPRETHERLLR
AFADAAARGSLQGLKALMAEDAELIGDGGGKVQTFSKSLHGSQRVAQLYFSLWRRMGPAV
RMEIVDINGEPGMLRFLDGELESAQTFEIEDGRIVRIRAQRNPDKLARIASLFSSK