Protein Info for GFF544 in Variovorax sp. SCN45

Annotation: Isoleucyl-tRNA synthetase (EC 6.1.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 926 TIGR00392: isoleucine--tRNA ligase" amino acids 15 to 841 (827 residues), 790.5 bits, see alignment E=1e-241 PF00133: tRNA-synt_1" amino acids 28 to 644 (617 residues), 416.6 bits, see alignment E=2.6e-128 PF09334: tRNA-synt_1g" amino acids 58 to 198 (141 residues), 36.5 bits, see alignment E=6.3e-13 PF08264: Anticodon_1" amino acids 688 to 844 (157 residues), 81.8 bits, see alignment E=1.2e-26 PF06827: zf-FPG_IleRS" amino acids 889 to 915 (27 residues), 22.3 bits, see alignment (E = 2.4e-08)

Best Hits

Swiss-Prot: 49% identical to SYI_AZOSB: Isoleucine--tRNA ligase (ileS) from Azoarcus sp. (strain BH72)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 49% identity to azo:azo1205)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.5

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (926 amino acids)

>GFF544 Isoleucyl-tRNA synthetase (EC 6.1.1.5) (Variovorax sp. SCN45)
MPDLKDTLNLLKTPFPMKGDLPTREPELVKRWAEQRVYERMRAAAAGRPPFILHDGPPYA
NGDIHIGHAVNKVLKDMVFKSRFFDGFDSQWIPGWDCHGMPIEHRIEQTNGRGLPTEQVQ
QLCRDYALQQTQSQKKDFLRLGLLGDWDRAFRTMDFETEANEIRFLDRIRQRGLLFRGQK
PVNWCVDCQSALAEAELEYAPKRSTTAYVGLLALDAVDFASRFKCQPMPGKQARLTIWTT
TPWTLPGNVAVGLDPRASYGLYDTPNGMLVMAAETGGKLLAGLGIDHELCAVAPGLDLVG
LRLKHPLLDTEVPLVAADFVSLDTGTGLVHLAPAHGAEDFELCRSLGIGGENVIDGAGRF
VAGLPMVGGMGLEEGSKLILDMLQADGSLLKQERIEHSYPHCWRHKTPILFRSTTQWFIG
MDRRATGDTRTLRETAREAIRDVPFYPASGRSRMEAMIDGRPDWCVSRQRTWGVPLPFFV
RRSDKSLHPQTARLVEEVALRIEREGISAWTRLKPADLGVDEAEYEKLSDTLDVWFDSGS
IHATVYRDARRPDAHGYPADLYLEGSDQHRGWFGSSLMTGCAADARAPFKAVLTHGFAVD
GDGKKMSKSLGNTVSPQQVASTRGADIIRLWIASTDYSTDISVSEEILDRVVEMYRRIRN
TIRFLLLNVSDFDATADCMRAQDLESVDQYAMLRCRELAEQCRKSYREYDFVAVTRLLHG
YCADELGGFYLDVLKDRLYASARDSRERRSAQTALHAILKNLLLLTAPILSFTAEEAWAV
LFKSQEDSVFVHIWDEAMPPRIDDSKVSEFWRQVRLLRPNVMKAIEDIRSSGAIGRSSEA
SIVIEAPVGIVESLALLGSELPSIFMVADVALKESDAVEVKVRRTSSLRCMRCWRHDATV
TDDAEQKMLCGRCRTVLSGLLELNDA