Protein Info for GFF5431 in Variovorax sp. SCN45
Annotation: Adenylosuccinate lyase (EC 4.3.2.2) @ SAICAR lyase (EC 4.3.2.2)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 61% identical to PUR8_PSEAE: Adenylosuccinate lyase (purB) from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
KEGG orthology group: K01756, adenylosuccinate lyase [EC: 4.3.2.2] (inferred from 97% identity to vap:Vapar_5167)MetaCyc: 61% identical to adenylosuccinate lyase (Escherichia coli K-12 substr. MG1655)
Adenylosuccinate lyase. [EC: 4.3.2.2]; 4.3.2.2 [EC: 4.3.2.2]
Predicted SEED Role
"Adenylosuccinate lyase (EC 4.3.2.2)" in subsystem De Novo Purine Biosynthesis or Purine conversions (EC 4.3.2.2)
MetaCyc Pathways
- superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
- superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
- superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
- superpathway of purine nucleotide salvage (12/14 steps found)
- inosine-5'-phosphate biosynthesis I (6/6 steps found)
- inosine-5'-phosphate biosynthesis II (5/5 steps found)
- superpathway of adenosine nucleotides de novo biosynthesis I (5/5 steps found)
- superpathway of adenosine nucleotides de novo biosynthesis II (6/7 steps found)
- adenosine ribonucleotides de novo biosynthesis (3/3 steps found)
- inosine-5'-phosphate biosynthesis III (5/6 steps found)
- dZTP biosynthesis (3/5 steps found)
KEGG Metabolic Maps
- Alanine and aspartate metabolism
- Biosynthesis of alkaloids derived from histidine and purine
- Purine metabolism
Isozymes
Compare fitness of predicted isozymes for: 4.3.2.2
Use Curated BLAST to search for 4.3.2.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Compare to protein structures
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (459 amino acids)
>GFF5431 Adenylosuccinate lyase (EC 4.3.2.2) @ SAICAR lyase (EC 4.3.2.2) (Variovorax sp. SCN45) MSFSTVSALSPLDGRYAAKLAALRPLMSEQGYMHRRVQVEVAWFIALSDCGFAEFKPLTG GARKYLLGLVSHFSEADALAIKEIEKTTNHDVKAVEYWIKSKFEARPELLAAAEFVHFAC TSEDINNTSHALQIQAARDKVVLPAIDGLIAKLREMAHQFANVSMLSRTHGQTASPTTVG KEIANVAVRLSKARAQIASVQLLGKMNGAVGNYNAHLAAWPEFDWEAFSRKVIETPAPLG LGLSFQPYSIQIEPHDYMAELFDAVARADTILIDFSRDIWGYVSLGYFKQRLKKGEIGSS TMPHKVNPIDFENAEGNLGLANALLRHLSEKLPISRWQRDLTDSTVLRNIGVAFGYATLA YASLATGLGKLELNEEALAEDLDASWEVLAEPIQTVMRRFGVQGAYEQLKEVTRGKTVTA EALHGLIRSLEIPEEEKARLLAMTPASYVGKAAELASRI