Protein Info for GFF5430 in Variovorax sp. SCN45

Annotation: Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 370 transmembrane" amino acids 12 to 38 (27 residues), see Phobius details amino acids 140 to 163 (24 residues), see Phobius details amino acids 186 to 208 (23 residues), see Phobius details amino acids 331 to 352 (22 residues), see Phobius details PF03929: PepSY_TM" amino acids 10 to 354 (345 residues), 169.1 bits, see alignment E=1e-53

Best Hits

KEGG orthology group: None (inferred from 80% identity to vap:Vapar_5168)

Predicted SEED Role

"Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (370 amino acids)

>GFF5430 Uncharacterized iron-regulated membrane protein; Iron-uptake factor PiuB (Variovorax sp. SCN45)
MRAPLRRLWLRIHRWIGLTLGPILALTALLGAMLVVALPLDRYAHPAFFTARSTGDAPLQ
LEPLRARLAAEFGPDANFTLRPPRRPGDTLWVMVRGQWEGTLYLDPTTGAEQGRRGTHEG
AYNLLFELHSSLLLEDTGKAILAVVALAYLFLLVTGLVLWWPVRWPPSLRIVLDRGLLRG
LFDLHRTGGAVLGLLIAVSVATGAYMAWRPLGNFISGLAGQQPVKPPVIAKGAATGPRLP
LDELVARAQQAFPGQPIGYIQVPGKTSRPMRVRFRLSDDPHPNGIGSVWLNTVTGEVLAA
RKWQDLDAGNGMVAVVYPLHTGELGGLLHEALTVLLGLALGGLGLSGVWLWWRRRAERRR
ARAPQIHSTT