Protein Info for GFF543 in Methylophilus sp. DMC18

Annotation: Flagellum-specific ATP synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 493 TIGR01026: ATPase, FliI/YscN family" amino acids 49 to 489 (441 residues), 589.3 bits, see alignment E=4.3e-181 TIGR03496: flagellar protein export ATPase FliI" amino acids 57 to 489 (433 residues), 653.2 bits, see alignment E=1.5e-200 PF00006: ATP-synt_ab" amino acids 200 to 411 (212 residues), 292.8 bits, see alignment E=1.5e-91 PF18269: T3SS_ATPase_C" amino acids 418 to 488 (71 residues), 96.7 bits, see alignment E=5.7e-32

Best Hits

KEGG orthology group: K02412, flagellum-specific ATP synthase [EC: 3.6.3.14] (inferred from 75% identity to meh:M301_1005)

Predicted SEED Role

"Flagellum-specific ATP synthase FliI" in subsystem Flagellar motility or Flagellum

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.14

Use Curated BLAST to search for 3.6.3.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (493 amino acids)

>GFF543 Flagellum-specific ATP synthase (Methylophilus sp. DMC18)
MTISDSGLPTLDAVADNSPQVTNEPAKQPNVHEQRWQSYLRDCSELINMVDPLEVAGRIV
KVTGLVMVATGIKMPIGGACFIPLQDGGRVEAEVVGFDGENLLLMPQTALEGVTPGAKVF
SMEIAETVPKPQIGRPPRRRAQDRARHFPVGDGLLGRVVDAAGNPLDGLGPIPGDVTAPL
NGRVINPLQRAPIEDTLDVGVRAINAMLTVGRGQRMGLFAGSGVGKSVLLGMMARYTTAD
VIVVGLIGERGREVQEFIEQILGPEGLARSVVVAAPADVSALMRLQGASYATAIAERFRQ
QGKNVLLIMDSLTRYAMAQREIALAIGEPPATKGYPPSVFAKLPALVERTGNGARGEGSI
TAFYTVLTEGDDQQDPIADSARAILDGHIVLNRSLAESGHYPAIDIEQSISRAMHNITSK
EHQQAARMLKQWYSRYLRNQDLIAVGAYQPGSDPMLDIAIRKRDDINAFLQQDIDECADM
QHSLQQLANTLTL