Protein Info for PS417_27735 in Pseudomonas simiae WCS417

Annotation: phospholipase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 522 signal peptide" amino acids 1 to 22 (22 residues), see Phobius details PF13091: PLDc_2" amino acids 71 to 223 (153 residues), 49.4 bits, see alignment E=4.1e-17 amino acids 324 to 463 (140 residues), 61.7 bits, see alignment E=6.7e-21 PF00614: PLDc" amino acids 417 to 441 (25 residues), 22.9 bits, see alignment (E = 6.9e-09)

Best Hits

KEGG orthology group: None (inferred from 96% identity to pfs:PFLU5974)

Predicted SEED Role

"Cardiolipin synthetase (EC 2.7.8.-)" in subsystem Glycerolipid and Glycerophospholipid Metabolism in Bacteria (EC 2.7.8.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.8.-

Use Curated BLAST to search for 2.7.8.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UJM2 at UniProt or InterPro

Protein Sequence (522 amino acids)

>PS417_27735 phospholipase (Pseudomonas simiae WCS417)
MIQRLLPFFLLGALTLGGCATVSTPRIPSDALPAAQSSFGRSIQAQAAPYQGRSGFRLLP
NSSEAFMARAELIRNAQISLDLQYYIVHDGISTRMLVDELLKAADRGVRVRILLDDTTSD
GLDQIIATLAAHPKIEIRLFNPLHLGRSTGATRAMGRLFNLSLQHRRMHNKLWLADNSVA
IVGGRNLGDEYFDAEPNLNFTDIDMLSVGPVAEQLGHSFDQYWNSALSKPIDDFVANMPS
KSDLAAARVRLEASLAESRQQNHALYNRLRTYQTQPRMDTWRRELIWAWNQALWDAPSKV
LAKADPDPRLLLTTQLAPELEGVNHELIMISAYFVPGQPGLVYLTGRADAGVSVSLLTNS
LEATDVPAVHGGYAPYRKALLEHGVKLYELRRQPGDPSGGSGPHLFRRGTFRGSDSSLHS
KAMIFDREKSFIGSFNFDPRSVLWNTEVGVLVDSPELAEHVRNLALQGMAPPLSYEAKLQ
DGQVVWVTEDNGQLHTLTREPGSWWRRFNAWFATSVGLERLL