Protein Info for GFF5415 in Hydrogenophaga sp. GW460-11-11-14-LB1

Annotation: Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 573 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details TIGR03075: PQQ-dependent dehydrogenase, methanol/ethanol family" amino acids 19 to 544 (526 residues), 741.6 bits, see alignment E=2.5e-227 PF13360: PQQ_2" amino acids 66 to 302 (237 residues), 62.9 bits, see alignment E=5.6e-21 amino acids 459 to 527 (69 residues), 36.1 bits, see alignment E=8.6e-13 PF01011: PQQ" amino acids 88 to 114 (27 residues), 21.8 bits, see alignment (E = 1.8e-08) amino acids 492 to 526 (35 residues), 36.2 bits, see alignment (E = 5.1e-13) PF13570: PQQ_3" amino acids 107 to 155 (49 residues), 18.1 bits, see alignment 4.5e-07 amino acids 469 to 508 (40 residues), 21.7 bits, see alignment 3.4e-08

Best Hits

Swiss-Prot: 51% identical to QEDH_PSEPU: Quinoprotein alcohol dehydrogenase (cytochrome c) (qedA) from Pseudomonas putida

KEGG orthology group: K00114, alcohol dehydrogenase (cytochrome c) [EC: 1.1.2.8] (inferred from 85% identity to pna:Pnap_0726)

MetaCyc: 52% identical to quinoprotein alcohol dehydrogenase (Pseudomonas putida U)
1.1.2.-

Predicted SEED Role

"Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8)" (EC 1.1.99.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.2.8, 1.1.99.8

Use Curated BLAST to search for 1.1.2.8 or 1.1.99.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (573 amino acids)

>GFF5415 Quino(hemo)protein alcohol dehydrogenase, PQQ-dependent (EC 1.1.99.8) (Hydrogenophaga sp. GW460-11-11-14-LB1)
VKRTLLSLMILAATAHVSAQGVTDAMIADDAKTPDNVLSWGLGTQGQRHSTLSGITERNI
NKLVPVWSMSFGGEKQRGQQSQPLVHNGKMFVTASYSRIYAVNTKTGEKLWKYEHRLPEG
IMPCCDVINRGAALYDNLVIFGTLDAQLVALDQNTGKVVWREKIDDYAAGYSYTAAPLIA
EGLLMTGVSGGEFGVVGRVEARDPRTGKMVWTRPVVEGHMGYKDGKENGITGTTNATWPG
ETWKTGGAATWLGGTYDAKTGLAYFGTGNPGPWNSHVRKGDNLYSASTVAIDVKTGKIVW
HYQSTPNDGWDYDGVNEFVTFDMDGKRMGAKADRNGFFYVNDAATGKLVNAFPFVKKVTW
ATGIDLKTGRPNFDDTNRPGDPTAAGGDGAKGKAVFSAPGFLGGKNQMPMAYSPKTGLFY
VPTNEWGMEIWNEPITYKKGAAFLGAGFTIKPLFDDHIGSLRAIDPKTGKVAWEVKNSAP
LWGGVLTAGDLVFWGTPEGYLKAADAKTGKVVWQFQTGSGVVAPPITWTEGGEQYVSVVS
GWGGAVPLWGGEVAKKVNFLEQGGSVWTFKLMK