Protein Info for GFF5410 in Variovorax sp. SCN45

Annotation: Adenylate cyclase (EC 4.6.1.1)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 signal peptide" amino acids 1 to 29 (29 residues), see Phobius details transmembrane" amino acids 379 to 399 (21 residues), see Phobius details amino acids 405 to 424 (20 residues), see Phobius details amino acids 430 to 453 (24 residues), see Phobius details PF05226: CHASE2" amino acids 27 to 390 (364 residues), 182.1 bits, see alignment E=1.6e-57 PF00211: Guanylate_cyc" amino acids 490 to 669 (180 residues), 113.7 bits, see alignment E=8.5e-37

Best Hits

KEGG orthology group: K01768, adenylate cyclase [EC: 4.6.1.1] (inferred from 82% identity to vpe:Varpa_5910)

Predicted SEED Role

"Adenylate cyclase (EC 4.6.1.1)" in subsystem cAMP signaling in bacteria (EC 4.6.1.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.6.1.1

Use Curated BLAST to search for 4.6.1.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (750 amino acids)

>GFF5410 Adenylate cyclase (EC 4.6.1.1) (Variovorax sp. SCN45)
VSVLRRHWPRIVISLLPVLLALAHATGAWPLSALERLDRLIYDIRLRATMPRTLDPRVVI
VDVDDASLARQGQWPWARDKIAGLTNELLGRQQVAVLGFDVMFLEPDRSSGLDSLRAMAA
GPLRGMQGLATEIERLAPTLDNDAAFAKSLESRPVALGYYFTRTGTPSAKGQLPAAPLLP
VDAFPAGGIYASSWNGFGASLPALAKAAPTAGFLNTLVGVQGDGAIRSVPLIARYEGDQA
QPGYYESLGLAVYRLVNGLPPVLPAFASGGAVPRLESLRVGRLRVPVDQMASMKVPFRGP
GGADGGSFRYIPAADVLEGRLGPGELKGKIVLVGATAPGLQDLRATPVEAAFPGVEVHAN
IVSGLLDHRLLSVPDYAPGYEVLTVLVTGLVLTFGLSLLSAPRAVLLVAGTIGALVGLNS
WLFMAHGLVLPLASGLAMTMLAFVLSMSWGYFVESRARRGLVRLFGTYVPPQLVDEMLVQ
PDRYSMRAESKPMTVMFCDMRDFTRLSEQMTPAQLQAFLNTVFSRLTDVISAHRGTVDKY
MGDCVMAFWGAPIDAPEHAALAVRAALDMADAVRDINEANRAGGRPEISVGIGINSGVMS
VGDMGSAARRSYTVVGDAVNLASRLEGLSGHYGVEIVASGATRELAPGYVWQELDSVRVK
GKAQAVAVFTPLAERTPEAEKQAQPLLERWGEVLAAYRRQDWAVGRNLLAPLLAADAKKV
LYQLYAQRLASMALRPQDPNWDGATRFETK