Protein Info for PS417_27660 in Pseudomonas simiae WCS417

Annotation: multidrug transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 467 transmembrane" amino acids 12 to 34 (23 residues), see Phobius details amino acids 54 to 77 (24 residues), see Phobius details amino acids 93 to 112 (20 residues), see Phobius details amino acids 131 to 149 (19 residues), see Phobius details amino acids 161 to 181 (21 residues), see Phobius details amino acids 192 to 214 (23 residues), see Phobius details amino acids 243 to 268 (26 residues), see Phobius details amino acids 275 to 298 (24 residues), see Phobius details amino acids 319 to 338 (20 residues), see Phobius details amino acids 357 to 374 (18 residues), see Phobius details amino acids 395 to 417 (23 residues), see Phobius details amino acids 424 to 443 (20 residues), see Phobius details TIGR00797: MATE efflux family protein" amino acids 20 to 425 (406 residues), 224.3 bits, see alignment E=1.2e-70 PF01554: MatE" amino acids 20 to 177 (158 residues), 89.2 bits, see alignment E=1.2e-29 amino acids 246 to 412 (167 residues), 99.4 bits, see alignment E=9.1e-33

Best Hits

Swiss-Prot: 72% identical to NORM_PSESM: Probable multidrug resistance protein NorM (norM) from Pseudomonas syringae pv. tomato (strain ATCC BAA-871 / DC3000)

KEGG orthology group: K03327, multidrug resistance protein, MATE family (inferred from 96% identity to pfs:PFLU5959)

Predicted SEED Role

"Multidrug and toxin extrusion (MATE) family efflux pump YdhE/NorM, homolog"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A1N7UE93 at UniProt or InterPro

Protein Sequence (467 amino acids)

>PS417_27660 multidrug transporter (Pseudomonas simiae WCS417)
MIMQHPARTELWAILRLSGPLIASQLAHMLMVLTDTLMMARLSPEALAGGGLGAASYSFV
SIFCIGVIAAVGTLVAIRHGAGDIEGATRLTQAGLWLAWLMALAAGLLLWNLKPLLLLFG
QTETNVHSAGQFLTILPFALPGFLSFMALRGFTSAIGKAQPVMVISLGGAVANYLLNHAL
IEGMFGLPKLGLMGIGLVTAVVANGMALALMWYIHRNRTYAAYPLSTGLLRLNTQYLREL
WRLGLPIGGTYAVEVGLFAFAALCMGTMGSTQLAAHQIALQIVSVAFMVPAGMSYAVTMR
IGQHYGAGQLLQARLAGRVGIGFGAAVMLGFAAVLWLYSDPLIGLLIDHNDPAFHDVIVL
AVSLLAVAAWFELFDGVQTIAMGCIRGLKDAKTTFLIGAGCYWLIGAPSAWLMAFTLGWG
PTGVWWGLALGLACAAVSLTWAFEAKMKRMIRREPETYSTLQAAQPD