Protein Info for GFF5369 in Variovorax sp. SCN45

Annotation: Large-conductance mechanosensitive channel

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 142 transmembrane" amino acids 16 to 50 (35 residues), see Phobius details amino acids 54 to 64 (11 residues), see Phobius details amino acids 84 to 106 (23 residues), see Phobius details PF01741: MscL" amino acids 3 to 141 (139 residues), 153.7 bits, see alignment E=1.5e-49 TIGR00220: large conductance mechanosensitive channel protein" amino acids 4 to 141 (138 residues), 136.5 bits, see alignment E=2.8e-44

Best Hits

Swiss-Prot: 88% identical to MSCL_VARPS: Large-conductance mechanosensitive channel (mscL) from Variovorax paradoxus (strain S110)

KEGG orthology group: K03282, large conductance mechanosensitive channel (inferred from 91% identity to vpe:Varpa_1275)

Predicted SEED Role

"Large-conductance mechanosensitive channel" in subsystem Potassium homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

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Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

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Protein Sequence (142 amino acids)

>GFF5369 Large-conductance mechanosensitive channel (Variovorax sp. SCN45)
MSILSEFKEFAVKGNVIDLAVGVIIGGAFGKIVDSLVADIIMPLVGLVFGKLDFSNLYVV
LGTAPAGVANTLADLKKAGVPVLAYGNFITIAVNFVILAFIIFMMVKQINRLKRSEEPAP
AAAPATPEDVILLREIRDSLKR