Protein Info for GFF5351 in Variovorax sp. SCN45

Annotation: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00007: 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase
  • amino acids 3 to 235 (233 residues), 259.7 bits, E=1.2e-81
• PF00977: His_biosynth
  • amino acids 3 to 232 (230 residues), 281 bits, E=6.6e-88

Best Hits

Swiss-Prot: 97% identical to HIS4_VARPS: 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase (hisA) from Variovorax paradoxus (strain S110)

KEGG orthology group: K01814, phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase [EC: 5.3.1.16] (inferred from 97% identity to vap:Vapar_1170)

Predicted SEED Role

"Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16)" in subsystem Histidine Biosynthesis (EC 5.3.1.16)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (43/46 steps found)
• L-histidine biosynthesis (10/10 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Histidine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.3.1.16

Protein Sequence (246 amino acids)

>GFF5351 Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase (EC 5.3.1.16) (Variovorax sp. SCN45)
MLLIPAIDLKDGHCVRLKQGDMDQSTVFSEDPAAMARKWVEAGARRLHLVDLNGAFAGKP
QNHAAIKAILKEVGDDIPVQLGGGIRDLDTIERYIDDGLRYVIIGTAAVKNPGFLKDACS
AFGGHIIVGLDAKDGKVATDGWSKLTGHEVADLGKKFEDYGVESIIYTDIGRDGMLSGIN
IEATVRLAQALTIPVIASGGLSNMADIDQLCAVESDGIEGVICGRAIYSGDLDFAAAQAR
ADELAA