Protein Info for GFF5345 in Variovorax sp. SCN45

Annotation: Histidinol dehydrogenase (EC 1.1.1.23)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 440 PF00815: Histidinol_dh" amino acids 29 to 437 (409 residues), 559.8 bits, see alignment E=2.1e-172 TIGR00069: histidinol dehydrogenase" amino acids 39 to 436 (398 residues), 523.8 bits, see alignment E=1.7e-161

Best Hits

Swiss-Prot: 73% identical to HISX_BURL3: Histidinol dehydrogenase (hisD) from Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)

KEGG orthology group: K00013, histidinol dehydrogenase [EC: 1.1.1.23] (inferred from 96% identity to vap:Vapar_1165)

Predicted SEED Role

"Histidinol dehydrogenase (EC 1.1.1.23)" in subsystem Histidine Biosynthesis (EC 1.1.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (440 amino acids)

>GFF5345 Histidinol dehydrogenase (EC 1.1.1.23) (Variovorax sp. SCN45)
MTLKAAPARLSTTSASFDAEFKARLHWSADDDAAIEKVVADILADVRKRGDEAVLEYTNR
FDKLDAKTLPELELTQAELKAAFESLPAAQRDALEAAARRVRSYHEAQKKASGESWSYRD
ADGTLLGQKVTPLDRVGIYVPGGKAAYPSSVLMNAIPAHVAGVGEIIMVVPTPKGEKNQL
VLAAAYVAGVTRGFTIGGAQAVAALAYGTATVPAVDKITGPGNAYVAAAKRRVFGTVGID
MIAGPSEILVLADGSTPPDWVAMDLFSQAEHDELAQSILLCPDAAYIDRVQAEIDRLLPA
MPRAEIIAASLNGRGALIHTKSMEEACEISNRIAPEHLEVSSSQPDRWEPLLRHAGAIFL
GAFTSESLGDYCAGPNHVLPTSGTARFSSPLGVYDFQKRSSLIEVSEAGAQVLGPIAVTL
AEGEGLQAHAEAARMRLRKI